First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

Autor: Ingrida Jaceviciene, D Boncea, K. Mahar, S Moore, Peter Hostnik, T Nokireki, S. Mrenoski, P Giraud, E Wodak, Miroslav Mojzis, D David, Ernest Ngoepe, Marcin Smreczak, Bertel Strandbygaard, Florence Cliquet, S Kenklies, M Nishimura, L B Chaves, Thomas Müller, Tomislav Bedeković, A Baur, P De Benedictis, I Almeida, M. Dobrostana, Marine Wasniewski, N Pejovic, Matthias König
Přispěvatelé: Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratorio Nacional de Investigacao Veterinaria (LNIV), Vet Med Labor GmbH, Croatian Veterinary Institute Laboratory for Rabies/Virology, Institute for Diagnosis and Animal Health, Laboratório de Diagnóstico da Raiva, Instituto Pasteur, Kimron Veterinary Institute Rabies Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Institute of Food Safety, Animal Health and Environment (BIOR), Laboratoire Départemental d'Analyses du Pas-de-Calais, National Veterinary Institute [Uppsala] (SVA), National Food and Veterinary Risk Assessment Institute of Lithuania, Landesamt für Verbraucherschutz Sachsen-Anhalt, Fachbereich Veterinärmedizin, Justus-Liebig-Universität Gießen (JLU), Estonian Veterinary and Food Laboratory, State Veterinary Institute Zvolen, Kansas State University, Ss. Cyril and Methodius University in Skopje, Friedrich-Loeffler-Institut (FLI), Agricultural Research Council-Onderstepoort Veterinary Institute, Research Institute for Animal Science In Biochemistry and Toxicology, Finnish Food safety Authority, Diagnostic Veterinary Laboratory Montenegro, National Veterinary Research Institute [Pulawy, Pologne] (NVRI), Danmarks Tekniske Universitet (DTU), Institute for Veterinary Disease Control Mödling (AGES)
Rok vydání: 2016
Předmět:
0301 basic medicine
Veterinary medicine
Oral vaccination campaigns
Rabies
International Cooperation
[SDV]Life Sciences [q-bio]
Concordance
030106 microbiology
Administration
Oral
Foxes
Animals
Wild
Enzyme-Linked Immunosorbent Assay
Antibodies
Viral
Sensitivity and Specificity
Rabies vaccination
Serology
03 medical and health sciences
Virology
parasitic diseases
medicine
Animals
Collaborative study
Disease surveillance
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Immunization Programs
business.industry
Reproducibility of Results
Raccoon Dogs
medicine.disease
3. Good health
Vaccination
030104 developmental biology
Rabies Vaccines
Rabies virus
Rabies antibodies
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
ELISA
Reagent Kits
Diagnostic
business
Antibody detection
Zdroj: Journal of Virological Methods
Journal of Virological Methods, Elsevier, 2016, 238, pp.77-85. ⟨10.1016/j.jviromet.2016.10.006⟩
ISSN: 0166-0934
Popis: International audience; The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.
Databáze: OpenAIRE
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