Mesenchymal stem cells in combination with erythropoietin repair hyperoxia-induced alveoli dysplasia injury in neonatal mice via inhibition of TGF-β1 signaling
| Autor: | Sun Chao, Yun Luan, Luan Zhang, Kaili Li, Zhaohua Zhang, Yi-Biao Wang, Xiaoli Liu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
MSCs Mice 0302 clinical medicine Fibrosis hemic and lymphatic diseases TGF-β1 Cells Cultured Bronchopulmonary Dysplasia Hyperoxia Hematology BPD respiratory system Combined Modality Therapy Recombinant Proteins medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Female medicine.symptom medicine.drug Signal Transduction Research Paper medicine.medical_specialty Epithelial-Mesenchymal Transition Mesenchymal Stem Cell Transplantation Transforming Growth Factor beta1 03 medical and health sciences Internal medicine medicine Animals Humans Erythropoietin business.industry Mesenchymal stem cell medicine.disease Mice Inbred C57BL Pulmonary Alveoli Disease Models Animal ETM 030104 developmental biology Bronchopulmonary dysplasia Dysplasia Immunology Cancer research Bone marrow business EPO |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Yun Luan 1 , Luan Zhang 2 , Sun Chao 1 , Xiaoli Liu 3 , Kaili Li 1 , Yibiao Wang 2 , Zhaohua Zhang 2 1 Central Research Laboratory, The Second Hospital of Shandong University, Jinan, China 2 Department of Pediatrics, The Second Hospital of Shandong University, Jinan, China 3 Department of Hematology, The Second Hospital of Shandong University, Jinan, China Correspondence to: Yibiao Wang, e-mail: wangyibiao@sdu.edu.cn Zhaohua Zhang, e-mail: zhaohuaz1972@126.com Keywords: BPD, MSCs, EPO, TGF-β1, ETM Received: December 09, 2015 Accepted: April 11, 2016 Published: May 12, 2016 ABSTRACT The aim of the present study is to investigate the protection effects of bone marrow mesenchymal stem cells (MSCs) in combination with EPO against hyperoxia-induced bronchopulmonary dysplasia (BPD) injury in neonatal mice. BPD model was prepared by continuous high oxygen exposure, 1×10 6 bone marrow MSCs and 5000U/kg recombinant human erythropoietin (EPO) were injected respectively. Results showed that administration of MSCs, EPO especially MSCs+EPO significant attenuated hyperoxia-induced lung damage with a decrease of fibrosis, radical alveolar counts and inhibition of the occurrence of epithelial-mesenchymal transition (EMT). Furthermore, MSCs+EPO co-treatment more significantly suppressed the levels of transforming growth factor-β1(TGF-β1) than MSCs or EPO alone. Collectively, these results suggested that MSCs, EPO in particular MSCs+EPO co-treatment could promote lung repair in hyperoxia-induced alveoli dysplasia injury via inhibition of TGF-β1 signaling pathway to further suppress EMT process and may be a promising therapeutic strategy. |
| Databáze: | OpenAIRE |
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