Native whey induces similar post exercise muscle anabolic responses as regular whey, despite greater leucinemia, in elderly individuals
| Autor: | Matthew Cotter, Elisabet Børsheim, Ina Garthe, Haakon B. Benestad, Håvard Hamarsland, Anne Lene Nordengen, Truls Raastad, Kari Holte, Sigve Nyvik Aas, Gøran Paulsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
protein quality Whey protein medicine.medical_specialty Anabolism 030309 nutrition & dietetics Muscle Proteins Medicine (miscellaneous) stable isotopes Article 03 medical and health sciences 0302 clinical medicine fluids and secretions Leucine Whey Internal medicine Animals Humans Medicine 030212 general & internal medicine skeletal muscle Exercise Aged 0303 health sciences Meal amino acids Cross-Over Studies Nutrition and Dietetics business.industry Skeletal muscle food and beverages Crossover study Whey Proteins Endocrinology medicine.anatomical_structure Dietary Supplements supplementation Phosphorylation Female Geriatrics and Gerontology business Protein quality |
| Zdroj: | The Journal of Nutrition, Health & Aging |
| ISSN: | 1279-7707 |
| Popis: | Objective Elderly muscle seems less sensitive to the anabolic stimulus of a meal. Changes in blood concentrations of leucine are suggested as one important trigger of the anabolic response in muscle. The aim of this study was to investigate whether native whey protein, containing high amounts of leucine, may be a more potent stimulator of muscle protein synthesis (MPS) in elderly than regular whey protein (WPC-80) or milk. Design Randomized controlled partial crossover. Setting Norwegian School of Sport Sciences. Participants 21 healthy elderly men and women (≥70 years). Intervention Participants received either 20 g of WPC-80 and native whey (n = 11) on separate days in a crossover design, or milk (n = 10). Supplements were ingested immediately and two hours after a bout of lower body heavy-load resistance exercise. Measurements Blood samples and muscle biopsies were collected to measure blood concentrations of amino acids by gas-chromatography mass spectrometry (GCMS), phosphorylation of p70S6K, 4E-BP1 and eEF-2 by immunoblotting and mixed muscle fractional synthetic rate (FSR) by use of [2H5]phenylalanine-infusion, GCMS and isotope-ratio mass spectrometry. Results Native whey increased blood leucine concentrations more than WPC-80 (P < 0.05), but not p70S6K phosphorylation or mixed muscle FSR. Both whey supplements increased blood leucine concentrations (P < 0.01) and P70S6K phosphorylation more than milk (P = 0.014). Native whey reached higher mixed muscle FSR values than milk (P = 0.026) 1-3h after exercise. Conclusions Despite greater increases in blood leucine concentrations than WPC-80 and milk, native whey was only superior to milk concerning increases in MPS and phosphorylation of P70S6K during a 5-hour post-exercise period in elderly individuals. Electronic Supplementary Material Supplementary material is available for this article at 10.1007/s12603-018-1105-6 and is accessible for authorized users. |
| Databáze: | OpenAIRE |
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