Inflammasome genes polymorphisms may influence the development of hepatitis C in the Amazonas, Brazil

Autor: Nadja Pinto Garcia, Juliana dos Santos Affonso, Aya Sadahiro, Jéssica Albuquerque da Silva, Adriana Malheiro, Hanna Lara Silva Negreiros Dray, Rajendranath Ramasawmy, Mauricio Morishi Ogusku, Flamir da Silva Victoria, Diana Mota Toro, Grenda Leite Pereira, Pedro Vieira da Silva Neto, Allyson Guimarães Costa, Keyla Santos de Sousa, Marilú Victória Barbieri, Eduardo Antônio Donadi, Andréa Monteiro Tarragô, Priscila Santos Sarmento
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Male
Linkage disequilibrium
Heredity
Gastroenterology and hepatology
Inflammasomes
Physiology
Interleukin-1beta
Hepacivirus
medicine.disease_cause
Biochemistry
Homozygosity
Hepatitis
Cathepsin B
Gene Frequency
Fibrosis
Polymorphism (computer science)
Immune Physiology
Genotype
Medicine
Pathology and laboratory medicine
Innate Immune System
Multidisciplinary
Immune System Proteins
Heterozygosity
Hepatitis C virus
Interleukin-18
Hepatitis C
Medical microbiology
Middle Aged
Neoplasm Proteins
DNA-Binding Proteins
Infectious hepatitis
Viruses
Infectious diseases
Cytokines
Interleukin 18
Female
Pathogens
Brazil
Research Article
Medical conditions
Adult
Science
Immunology
Viral diseases
Microbiology
Polymorphism
Single Nucleotide
Young Adult
NLR Family
Pyrin Domain-Containing 3 Protein
Genetics
Humans
Genetic Predisposition to Disease
Allele
Alleles
Liver diseases
Aged
Medicine and health sciences
Flaviviruses
business.industry
GENÓTIPOS
Organisms
Viral pathogens
Biology and Life Sciences
Proteins
Molecular Development
medicine.disease
Hepatitis viruses
Microbial pathogens
CARD Signaling Adaptor Proteins
Genetic Loci
Immune System
business
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 6, p e0253470 (2021)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1932-6203
Popis: Hepatitis C is considered a major public health problem caused by the hepatitis C virus (HCV). Viral infections are known to induce production of IL1β through the signaling pathway of inflammasomes. Emerging evidences suggest that Inflammasome genes may influence the immune response against HCV as the host genetic background may contribute to the balance between acute and chronic inflammation. We investigated in 151 patients with chronic hepatitis C and 206 healthy blood donors’ individuals (HD). Polymorphisms in the IL1B and IL18 genes were genotyped by PCR-RFLP, while NLRP3, CARD8, CTSB and AIM2 by RT- PCR. Serum assay of IL-1β cytokine was performed by ELISA. 84 patients presented mild fibrosis (NLRP3-rs10754558 C/C genotype correlated with higher IL-1β levels compared to the G/G genotype. Similar pattern was observed in patients with hepatitis C, mean circulating IL-1β levels were 21,96 ± 4.5 and 10,62 ± 3.3pg/mL among the C/C and G/G genotypes, respectively. This pattern holds even after stratification of the patients into mild fibrosis and advanced fibrosis, demonstrating that the NLRP3-rs10754558 or another polymorphism in linkage disequilibrium with it possibly has an influence on the processing of pro-IL-1β. Notably, higher levels of IL-1β (Mann–Whitney test, pCARD8-rs2009373 and IL1B-rs16944 are less prone to hepatitis C development (padj = 0.039). Similarly, herozygote carriers for CTSB-rs1692816 and AIM2-rs1103577 (padj = 0.008) or for IL18-rs187238 and NLRP3-rs10754558 (padj = 0.005), have less chances to the development of hepatitis C. However, between subgroups of CARD8-rs2009373 and heterozygous for IL18-rs187238 (padj = 0.028), have mild form of fibrosis.
Databáze: OpenAIRE
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