Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Autor: Shifaq Kamili, Kenneth S. Plante, Yan Li, Rashi Gautam, Craig Schindewolf, Lijuan Wang, Krishna Narayanan, Hannah A. Bullock, Janna Murray, Brett Whitaker, Brian Lynch, Natalie J. Thornburg, Azaibi Tamin, Cynthia S. Goldsmith, Scott C. Weaver, Shinji Makino, Clinton R. Paden, Stephen Lindstrom, Dionna Scharton, Ying Tao, Kumari G. Lokugamage, Anna Uehara, Jing Zhang, Krista Queen, Senthil Kumar K. Sakthivel, Steven G. Widen, Vineet D. Menachery, Haibin Wang, Xiaoyan Lu, Suxiang Tong, Thomas G. Ksiazek, Divya Mirchandani, Jennifer L Harcourt, Jessica A. Plante
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Epidemiology
Expedited
viruses
coronavirus
Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with 2019 Novel Coronavirus Disease
United States

Oropharynx
lcsh:Medicine
medicine.disease_cause
Virus Replication
0302 clinical medicine
Nasopharynx
Pandemic
Chlorocebus aethiops
Medicine
characterization
030212 general & internal medicine
Coronavirus
biology
Infectious Diseases
PCR
Coronavirus Infections
isolation
severe acute respiratory syndrome coronavirus 2
Microbiology (medical)
Washington
030231 tropical medicine
Pneumonia
Viral

Genome
Viral

Virus
2019 novel coronavirus disease
Cell Line
lcsh:Infectious and parasitic diseases
03 medical and health sciences
respiratory infections
Betacoronavirus
Animals
Humans
lcsh:RC109-216
Pandemics
Vero Cells
business.industry
SARS-CoV-2
Research
lcsh:R
Outbreak
COVID-19
biology.organism_classification
medicine.disease
Virology
United States
zoonoses
Pneumonia
Viral Tropism
novel coronavirus disease 2019
Tissue tropism
Vero cell
business
Zdroj: Emerging Infectious Diseases, Vol 26, Iss 6, Pp 1266-1273 (2020)
Emerging Infectious Diseases
ISSN: 1080-6059
1080-6040
Popis: The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.
Databáze: OpenAIRE