Metastatic Melanoma Imaging with an 111In-labeled Lactam Bridge-cyclized Alpha-Melanocyte Stimulating Hormone Peptide
| Autor: | Haixun Guo, Nalini Shenoy, Benjamin M. Gershman, Larry A. Sklar, Jianquan Yang, Yubin Miao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Biodistribution Pathology medicine.medical_specialty Time Factors Lactams media_common.quotation_subject Peptide Binding Competitive Peptides Cyclic Article chemistry.chemical_compound Heterocyclic Compounds 1-Ring Mice Fluorodeoxyglucose F18 Cell Line Tumor medicine Animals Radiology Nuclear Medicine and imaging Tissue Distribution Amino Acid Sequence Neoplasm Metastasis Internalization neoplasms Melanoma media_common Neoplasm Staging chemistry.chemical_classification Tomography Emission-Computed Single-Photon medicine.diagnostic_test Staining and Labeling Indium Radioisotopes Reproducibility of Results medicine.disease chemistry Positron emission tomography Cell culture alpha-MSH Cancer research Lactam Molecular Medicine Feasibility Studies Tomography X-Ray Computed Emission computed tomography |
| Popis: | The purpose of this study was to examine whether a novel lactam bridge-cyclized (111)In-labeled 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid-Gly-Glu-c[Lys-Nle-Glu-His-d-Phe-Arg-Trp-Gly-Arg-Pro-Val-Asp] {DOTA-GlyGlu-CycMSH} could be an effective imaging probe for metastatic melanoma detection.(111)In-DOTA-GlyGlu-CycMSH was prepared and purified by reverse-phase high-performance liquid chromatography (RP-HPLC). The internalization and efflux of (111)In-DOTA-GlyGlu-CycMSH were examined in B16/F10 melanoma cells. The biodistribution of (111)In-DOTA-GlyGlu-CycMSH was determined in B16/F10 pulmonary metastatic melanoma-bearing and normal C57 mice. Pulmonary metastatic melanoma imaging was performed by small-animal single-photon emission computed tomography (SPECT)/CT (Nano-SPECT/CT) using (111)In-DOTA-GlyGlu-CycMSH as an imaging probe and compared with 2-[(18)F]fluoro-2-deoxy-d-glucose ([(18)F]FDG) positron emission tomography (PET) imaging.(111)In-DOTA-GlyGlu-CycMSH was readily prepared with greater than 95% radiolabeling yield. (111)In-DOTA-GlyGlu-CycMSH displayed rapid internalization and extended efflux in B16/F10 cells. (111)In-DOTA-GlyGlu-CycMSH exhibited significantly (P.05) higher uptakes (2.00+/-0.74%ID/g at 2 h post-injection and 1.83+/-0.12%ID/g at 4 h post-injection) in metastatic melanoma-bearing lung than that in normal lung (0.08+/-0.08%ID/g and 0.05+/-0.05%ID/g at 2 and 4 h post-injection, respectively). The activity accumulation in normal organs was low (0.5%ID/g) except for the kidneys 2 and 4 h post-injection. B16/F10 pulmonary melanoma metastases were clearly visualized with (111)In-DOTA-GlyGlu-CycMSH 2 h post-injection rather than with [(18)F]FDG 1 h post-injection.(111)In-DOTA-GlyGlu-CycMSH exhibited favorable metastatic melanoma-targeting and -imaging properties, highlighting its potential as an effective imaging probe for metastatic melanoma detection. |
| Databáze: | OpenAIRE |
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