Response surface optimization of D(-)-lactic acid production by Lactobacillus SMI8 using corn steep liquor and yeast autolysate as an alternative nitrogen source
| Autor: | Luciana Fontes Coelho, Cristian J. Bolner de Lima, Jonas Contiero, Kate Cristina Blanco |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Central composite design
Applied Microbiology and Biotechnology Corn steep liquor law.invention response surface methodology chemistry.chemical_compound Erlenmeyer flask law Lactobacillus Genetics yeast autolysate Food science Response surface methodology Molecular Biology D(-)-Lactic acid Lactobacillus corn steep liquor yeast autolysate response surface methodology medium optimization biology Chemistry food and beverages biology.organism_classification D(-)-Lactic acid Yeast Lactic acid Biochemistry medium optimization corn steep liquor Fermentation Agronomy and Crop Science Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 8, No 21 (2009) Scopus-Elsevier Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1684-5315 |
| Popis: | Made available in DSpace on 2013-08-28T14:09:01Z (GMT). No. of bitstreams: 1 WOS000273718700031.pdf: 214478 bytes, checksum: 334aeebb3a6f52f90e8f930db54c7824 (MD5) Made available in DSpace on 2013-09-30T18:47:38Z (GMT). No. of bitstreams: 2 WOS000273718700031.pdf: 214478 bytes, checksum: 334aeebb3a6f52f90e8f930db54c7824 (MD5) WOS000273718700031.pdf.txt: 22061 bytes, checksum: 78fa2d8a29ec4da8ef20ea46ccc0b726 (MD5) Previous issue date: 2009-11-02 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:56:30Z No. of bitstreams: 2 WOS000273718700031.pdf: 214478 bytes, checksum: 334aeebb3a6f52f90e8f930db54c7824 (MD5) WOS000273718700031.pdf.txt: 22061 bytes, checksum: 78fa2d8a29ec4da8ef20ea46ccc0b726 (MD5) Made available in DSpace on 2014-05-20T13:56:30Z (GMT). No. of bitstreams: 2 WOS000273718700031.pdf: 214478 bytes, checksum: 334aeebb3a6f52f90e8f930db54c7824 (MD5) WOS000273718700031.pdf.txt: 22061 bytes, checksum: 78fa2d8a29ec4da8ef20ea46ccc0b726 (MD5) Previous issue date: 2009-11-02 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The production of D(-) lactic acid from Lactobacillus LMI8 sp. was studied, using 2 low-cost nitrogen sources: corn steep liquor (CSL) and yeast autolysate (YA). A central composite design was used to determine maximal lactic acid production. The surface response method was used to evaluate the results of the experiments. The assays were performed in 250 mL Erlenmeyer flasks containing 100 mL of production medium maintained under refrigerated incubation at 200 rpm and a temperature of 37 +/- 1 degrees C for 48 h of fermentation. Lactic acid production was significantly affected by the isolated CSL as well as the interaction between CSL and YA. Maximal production of D(+) lactic acid was 41.42 g/L - a value located at the central point, which corresponded to 15 g/L of CSL and 5 g/L of YA. UNESP Rio Claro, São Paulo State Univ, Dept Biochem & Microbiol, Inst Biol Sci, BR-13506900 Rio Claro, SP, Brazil UNESP Rio Claro, São Paulo State Univ, Dept Biochem & Microbiol, Inst Biol Sci, BR-13506900 Rio Claro, SP, Brazil |
| Databáze: | OpenAIRE |
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