Biological Characterization of Polymeric Matrix and Graphene Oxide Biocomposites Filaments for Biomedical Implant Applications: A Preliminary Report
| Autor: | Thamires Santos Silva, Ana Claudia Oliveira Carreira, Michelle Silva Araujo, Gustavo de Sá Schiavo Matias, Maria Angélica Miglino, Hianka Jasmyne Costa de Carvalho, Carolina Coming Tegon, Gustavo Henrique Doná Rodrigues Almeida, Paula Fratini, Marcelo Melo Soares, Andressa de Aguiar Oliveira, Marcos Massi, Leandro Norberto da Silva Júnior |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Polymers and Plastics Biocompatibility Scanning electron microscope Oxide Organic chemistry Article law.invention Nanomaterials chemistry.chemical_compound biocompatibility QD241-441 Tissue engineering law nanomaterials mesenchymal stem cells poly (L-lactic acid) Nanocomposite Graphene Mesenchymal stem cell carbon nanosheets RATOS WISTAR General Chemistry chemistry tissue engineering Biomedical engineering |
| Zdroj: | Polymers, Vol 13, Iss 3382, p 3382 (2021) Polymers Volume 13 Issue 19 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2073-4360 |
| Popis: | Carbon nanostructures application, such as graphene (Gr) and graphene oxide (GO), provides suitable efforts for new material acquirement in biomedical areas. By aiming to combine the unique physicochemical properties of GO to Poly L-lactic acid (PLLA), PLLA-GO filaments were produced and characterized by X-ray diffraction (XRD). The in vivo biocompatibility of these nanocomposites was performed by subcutaneous and intramuscular implantation in adult Wistar rats. Evaluation of the implantation inflammatory response (21 days) and mesenchymal stem cells (MSCs) with PLLA-GO took place in culture for 7 days. Through XRD, new crystallographic planes were formed by mixing GO with PLLA (PLLA-GO). Using macroscopic analysis, GO implanted in the subcutaneous region showed particles’ organization, forming a structure similar to a ribbon, without tissue invasion. Histologically, no tissue architecture changes were observed, and PLLA-GO cell adhesion was demonstrated by scanning electron microscopy (SEM). Finally, PLLA-GO nanocomposites showed promising results due to the in vivo biocompatibility test, which demonstrated effective integration and absence of inflammation after 21 days of implantation. These results indicate the future use of PLLA-GO nanocomposites as a new effort for tissue engineering (TE) application, although further analysis is required to evaluate their proliferative capacity and viability. |
| Databáze: | OpenAIRE |
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