Effect of erythromycin treatment delay on therapeutic outcome of experimental acute otitis media caused by Streptococcus pneumoniae
| Autor: | Gema del Prado, Juan José Granizo, Carmina Martínez-Marín, Francisco Soriano, José I. García-Cía, Carlos Cenjor, Lorena Huelves, Carmen Ponte |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Time Factors medicine.drug_class Acute otitis media Antibiotics Erythromycin Microbial Sensitivity Tests Pharmacology Gerbil medicine.disease_cause Pneumococcal Infections Streptococcus pneumoniae medicine Animals Pharmacology (medical) Antibacterial agent Dose-Response Relationship Drug biology business.industry Streptococcaceae biology.organism_classification Anti-Bacterial Agents Disease Models Animal Otitis Media Treatment Outcome Infectious Diseases Otitis Immunology medicine.symptom Gerbillinae business medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 56:783-786 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dki322 |
| Popis: | OBJECTIVE To evaluate the effect of delayed administration of erythromycin in the course of acute otitis media caused by an erythromycin-susceptible Streptococcus pneumoniae strain in the gerbil model. METHODS The bacterium was inoculated by transbullar challenge in the middle ear (ME) and antibiotic treatment at different doses was administered at various times thereafter. RESULTS When 2.5 mg/kg of erythromycin was administered as a single dose 2, 5, 18 or 21 h post-inoculation (pi) the bacterial eradication rate was 55, 40, 0 and 0%, respectively. A higher dose (5 mg/kg) administered also as a single dose 2, 5, 18 and 21 h pi achieved bacterial eradication rates of 62.5, 43.8, 0 and 0%, respectively. Using a very high dose (50 mg/kg) repeated three times at 3 h intervals (total dose 150 mg/kg) and starting the treatment 21 h pi only achieved bacterial eradication in 25% of cases. The concentration of erythromycin achieved in the ME 90 min after administration of 5 mg/kg 5 or 21 h pi was very similar (0.74 and 0.79 mg/L) but the ME half-life was longer (98.2 min) with the early administration as compared with the delayed form (47.5 min), which could partially explain the different results. Further experiments showed that the failures observed with the delayed administration were not related to the time elapsed from antibiotic administration to ME sampling or selection of antibiotic-resistant mutants. CONCLUSION Bacteriological and clinical efficacies were significantly diminished if antibiotic administration was delayed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|