Porphyrin Binding and Irradiation Promote G-Quadruplex DNA Dimeric Structure
| Autor: | Aurelien Thureau, M. Longo, Anne Martel, Alessandro Paciaroni, Caterina Petrillo, Valeria Libera, Giorgio Schirò, Lucia Comez, Elena A. Andreeva |
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| Přispěvatelé: | Dipartimento di Fisica e Geologia [Perugia], Università degli Studi di Perugia (UNIPG), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), European Synchrotron Radiation Facility (ESRF), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Jülich Centre for Neutron Science (JCNS), Forschungszentrum Jülich GmbH at Heinz Maier-Leibnitz Zentrum (MLZ), Università degli Studi di Perugia = University of Perugia (UNIPG) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Porphyrins
Dimer MESH: Molecular Structure 010402 general chemistry G-quadruplex SEQUENCE 01 natural sciences MESH: Circular Dichroism Scattering 03 medical and health sciences chemistry.chemical_compound MESH: X-Rays ABSORPTION Scattering Radiation General Materials Science Physical and Theoretical Chemistry MESH: Scattering Radiation Protein secondary structure 030304 developmental biology 0303 health sciences MESH: Porphyrins Radiation Molecular Structure [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] Circular Dichroism X-Rays MESH: DNA CATIONIC PORPHYRINS DNA Telomere Porphyrin Small molecule 0104 chemical sciences G-Quadruplexes chemistry MESH: Dimerization Nucleic acid Biophysics Protein quaternary structure LIGAND MESH: Telomere TELOMERE G-QUADRUPLEX Dimerization TMPYP4 MESH: G-Quadruplexes |
| Zdroj: | Journal of Physical Chemistry Letters Journal of Physical Chemistry Letters, American Chemical Society, 2021, 12 (33), pp.8096-8102. ⟨10.1021/acs.jpclett.1c01840⟩ Journal of Physical Chemistry Letters, 2021, 12 (33), pp.8096-8102. ⟨10.1021/acs.jpclett.1c01840⟩ Libera, V, Andreeva, E A, Martel, A, Thureau, A, Longo, M, Petrillo, C, Paciaroni, A, Schiro, G & Comez, L 2021, ' Porphyrin Binding and Irradiation Promote G-Quadruplex DNA Dimeric Structure ', Journal of Physical Chemistry Letters, vol. 12, no. 33, pp. 8096-8102 . https://doi.org/10.1021/acs.jpclett.1c01840 |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/acs.jpclett.1c01840⟩ |
| Popis: | International audience; Nucleic acid sequences rich in guanines can organize into noncanonical DNA G-quadruplexes (G4s) of variable size. The design of small molecules stabilizing the structure of G4s is a rapidly growing area for the development of novel anticancer therapeutic strategies and bottom-up nanotechnologies. Among a multitude of binders, porphyrins are very attractive due to their light activation that can make them valuable conformational regulators of G4s. Here, a structure-based strategy, integrating complementary probes, is employed to study the interaction between TMPyP4 porphyrin and a 22-base human telomeric sequence (Tel22) before and after irradiation with blue light. Porphyrin binding is discovered to promote Tel22 dimerization, while light irradiation of the Tel22-TMPyP4 complex controls dimer fraction. Such a change in quaternary structure is found to be strictly correlated with modifications at the secondary structure level, thus providing an unprecedented link between the degree of dimerization and the underlying conformational changes in G4s. |
| Databáze: | OpenAIRE |
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