Identification and characterization of a LuxI/R-type quorum sensing system in Pseudoalteromonas
Autor: | Juanping Qiu, Zhiliang Yu, Jianhua Yin, Mengdan Ding, Dongliang Yu, Mengting Zhang, Shijun Wu, Jiadi Zhang, Yanfeng Mao |
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Rok vydání: | 2019 |
Předmět: |
Mutagenesis (molecular biology technique)
Sequence alignment Acyl-Butyrolactones medicine.disease_cause Microbiology 03 medical and health sciences Pseudoalteromonas Bacterial Proteins Drug Resistance Bacterial medicine Amino Acid Sequence Molecular Biology Escherichia coli 030304 developmental biology Genetics 0303 health sciences Acyl-Homoserine Lactones biology Base Sequence Whole Genome Sequencing 030306 microbiology food and beverages Quorum Sensing General Medicine Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Repressor Proteins Quorum sensing Streptomycin Trans-Activators Heterologous expression Sequence Alignment Bacteria Genome Bacterial Signal Transduction Transcription Factors |
Zdroj: | Research in microbiology. 170(6-7) |
ISSN: | 1769-7123 |
Popis: | Bacteria usually produce, release and detect quorum sensing (QS)-based signal molecules, and successively orchestrate gene expression in respond to environmental changes. Pseudoalteromonas are typical marine bacteria, but knowledge on their QS systems is extremely fragmentary. In this study, genome sequencing of Pseudoalteromonas sp. R3 was performed. Accordingly, a QS working model including three sets of hierarchically organized QS systems was proposed in strain R3. Among them, the typical LuxI/R-type QS system using acyl-homoserine lactones (AHLs) as signal molecules was characterized. Sequence similarity analysis indicated luxI encoding AHLs synthase is novel. The luxR encoding AHLs receptor is directly adjacent to luxI downstream. Notably, mutagenesis demonstrated LuxI and LuxR affect each other at transcriptional level, and both control the AHLs formation. Interestingly, it was found that LuxI/R-type QS system positively involves resistance to streptomycin. Thin-layer chromatography analysis showed strain R3 can produce 3-OH-C6-HSL and C8-HSL, which was supported by heterologous expression of LuxI in Escherichia coli. Sequence alignment analysis indicated that the N-terminal region of LuxI is more conservative than the C-terminal region, revealing the importance of N-terminal region in AHLs synthesis. The obtained findings enrich our knowledge on LuxI/R-type QS system in Pseudoalteromonas and its regulation on adaptation to marine environments. |
Databáze: | OpenAIRE |
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