The differential regulation of cell motile activity through matrix stiffness and porosity in three dimensional collagen matrices
Autor: | Joachim Seemann, Frederick Grinnell, Miguel Miron-Mendoza |
---|---|
Rok vydání: | 2010 |
Předmět: |
Morphology (linguistics)
Materials science Cell Biophysics Bioengineering Matrix (biology) Article Biomaterials Extracellular matrix Cell Movement medicine Humans Phosphorylation Cells Cultured Actin Rheometry Stiffness Cell migration Fibroblasts Actins Cell biology medicine.anatomical_structure Glutaral Mechanics of Materials Focal Adhesion Protein-Tyrosine Kinases Ceramics and Composites Collagen medicine.symptom Porosity |
Zdroj: | Biomaterials. 31:6425-6435 |
ISSN: | 0142-9612 |
DOI: | 10.1016/j.biomaterials.2010.04.064 |
Popis: | In three dimensional collagen matrices, cell motile activity results in collagen translocation, cell spreading and cell migration. Cells can penetrate into the matrix as well as spread and migrate along its surface. In the current studies, we quantitatively characterize collagen translocation, cell spreading and cell migration in relationship to collagen matrix stiffness and porosity. Collagen matrices prepared with 1-4 mg/ml collagen exhibited matrix stiffness (storage modulus measured by oscillating rheometry) increasing from 4 to 60 Pa and matrix porosity (measured by scanning electron microscopy) decreasing from 4 to 1 microm(2). Over this collagen concentration range, the consequences of cell motile activity changed markedly. As collagen concentration increased, cells no longer were able to cause translocation of collagen fibrils. Cell migration increased and cell spreading changed from dendritic to more flattened and polarized morphology depending on location of cells within or on the surface of the matrix. Collagen translocation appeared to depend primarily on matrix stiffness, whereas cell spreading and migration were less dependent on matrix stiffness and more dependent on collagen matrix porosity. |
Databáze: | OpenAIRE |
Externí odkaz: |