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Epicauta pestifera Werner ( Meloidae ) is a species of blister beetle which is found in North America and contains a powerful irritant and vesicant. This paper reports the isolation, quantitative determination, and identification of the active principle responsible for this activity. Employing standard practices of analysis, which involved infrared and NMR spectroscopy, thin-layer chromatography, elemental analysis, and determination of physical constants, the substance was identified as cantharidin. |
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