Tau complexes with phospholipase C-γ in situ
Autor: | Gail V.W. Johnson, Scott M. Jenkins |
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Rok vydání: | 1998 |
Předmět: |
Neurons
biology Phospholipase C Phospholipase C gamma General Neuroscience Tau protein tau Proteins Phospholipase Precipitin Tests In vitro Cell biology Isoenzymes Cell culture In vivo Type C Phospholipases mental disorders Tumor Cells Cultured biology.protein Humans Signal transduction Signal Transduction |
Zdroj: | NeuroReport. 9:67-71 |
ISSN: | 0959-4965 |
Popis: | Based on the results of recent in vitro studies, tau has been proposed to be involved in regulating signal transduction through the phospholipase C-gamma (PLC-gamma) signaling pathway. The present study provides support for the physiological relevance of this hypothesis by demonstrating the existence of a tau-PLC-gamma complex in situ in a human neuroblastoma cell line. Both PLC-gamma and PLC-delta, but not PLC-beta, co-purified with microtubule-associated proteins. PLC-gamma, but neither PLC-delta nor PLC-beta, co-immunoprecipitated with tau, and the PLC co-precipitating with tau was enzymatically active. Additionally, both tau and MAP-2 co-precipitated with PLC-gamma. These studies indicate that tau associates, either directly or indirectly, with PLC-gamma in situ, suggesting that tau may be appropriately localized to participate in the regulation of signal transduction through the PLC-gamma pathway in vivo. |
Databáze: | OpenAIRE |
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