Evaluation of novel platinum(II) based AIE compound-encapsulated mesoporous silica nanoparticles for cancer theranostic application
| Autor: | Nigam P. Rath, Clàudia Climent, Sheik Saleem Pasha, Inamur Rahaman Laskar, Leena Fageria, Rajdeep Chowdhury, Aniruddha Roy, Pere Alemany |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cell Survival
Aptamer Nanoparticle Antineoplastic Agents Apoptosis 02 engineering and technology 010402 general chemistry 01 natural sciences Theranostic Nanomedicine Inorganic Chemistry Cell Line Tumor Humans Molecule Cytotoxicity Càncer Platí Fluorescent Dyes Cancer Platinum Drug Carriers Nanopartícules Chemistry Cell Cycle Checkpoints Aptamers Nucleotide Mesoporous silica Silicon Dioxide 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Cancer cell Biophysics Nanoparticles 0210 nano-technology Porosity Intracellular |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona |
| Popis: | Advanced biomedical research has established that cancer is a multifactorial disorder which is highly heterogeneous in nature and responds differently to different treatment modalities, due to which constant monitoring of therapy response is becoming extremely important. To accomplish this, different theranostic formulations have been evaluated. However, most of them are found to suffer from several limitations extending from poor resolution, radiation damage, to high costs. In order to develop a better theranostic modality, we have designed and synthesized a novel platinum(ii)-based 'aggregation induced emission' (AIE) molecule (named BMPP-Pt) which showed strong intra-cellular fluorescence and also simultaneously exhibited potent cytotoxic activity. Due to this dual functionality, we wanted to explore the possibility of using this compound as a single molecule based theranostic modality. This compound was characterized using elemental analysis, NMR and IR spectroscopy, mass spectrometry and single crystal X-ray structure determination. BMPP-Pt was found to exhibit a high AIE property with emission maxima at 497 nm. For more efficient cancer cell targeting, BMPP-Pt was encapsulated into mesoporous silica nanoparticles (Pt-MSNPs) and the MSNPs were further surface modified with an anti-EpCAM aptamer (Pt-MSNP-E). Pt-MSNPs exhibited higher intracellular fluorescence compared to free BMPP-Pt, though both of them induced a similar degree of cell death via the apoptosis pathway, possibly via cell cycle arrest in the G1 phase. Anti-EpCAM aptamer modification was found to increase both cytotoxicity and intracellular fluorescence compared to unmodified MSNPs. Our study showed that EpCAM functionalized BMPP-Pt loaded MSNPs can efficiently internalize and induce apoptosis of cancer cells as well as show strong intracellular fluorescence. This study provides clues towards the development of a potential single compound based theranostic modality in future. |
| Databáze: | OpenAIRE |
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