Aqueous Fraction from Hibiscus sabdariffa Relaxes Mesenteric Arteries of Normotensive and Hypertensive Rats through Calcium Current Reduction and Possibly Potassium Channels Modulation

Autor: Véronique Maupoil, Elhadi M. Ahmed, Leslie Boudesocque-Delaye, Côme Pasqualin, Cécile Enguehard-Gueiffier, Anas M.A. Alsayed, Nicolas Peineau, Pierre Bredeloux, Bei Li Zhang, Angèle Yu
Přispěvatelé: Department of Pharmacognosy, Faculty of Pharmacy, University of Gezira, Signalisation et Transports Ioniques Membranaires (STIM), Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Pharmacologie, Université de Tours, Groupe Physiologie des Cellules Cardiaques et Vasculaires [Tours], Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Synthèse et isolement de molécules bio-actives EA 7502 (SIMBA), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Département de physiologie animale, Université Francois Rabelais [Tours], Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Patch-Clamp Techniques
Potassium Channels
Vasodilator Agents
[SDV]Life Sciences [q-bio]
Pharmacology
Rats
Inbred WKY
chemistry.chemical_compound
0302 clinical medicine
Rats
Inbred SHR
Mesenteric arteries
vasorelaxation
Nutrition and Dietetics
Hibiscus sabdariffa
anthocyanins
Potassium channel
Mesenteric Arteries
3. Good health
medicine.anatomical_structure
Hibiscus
030220 oncology & carcinogenesis
cardiovascular system
Ca2+ current
lcsh:Nutrition. Foods and food supply
medicine.drug
hypertension
mesenteric artery
lcsh:TX341-641
Flowers
Article
Contractility
03 medical and health sciences
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Potassium Channel Blockers
medicine
[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Animals
Patch clamp
Phenylephrine
Antihypertensive Agents
Tetraethylammonium
Plant Extracts
Membrane hyperpolarization
Rats
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
030104 developmental biology
chemistry
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Calcium Channels
Food Science
Zdroj: Nutrients, Vol 12, Iss 1782, p 1782 (2020)
Nutrients
Nutrients, MDPI, 2020, 12 (6), pp.E-1782. ⟨10.3390/nu12061782⟩
Volume 12
Issue 6
ISSN: 2072-6643
Popis: Background/Objectives: Hibiscus sabdariffa L. (H. sabdariffa (HS)) extract has a vascular relaxant effect on isolated rat thoracic aorta, but data on small resistance arteries, which play an important role on the development of hypertension, are still missing. The purposes of this study were (1) to assess the effect on isolated mesenteric arteries (MA) from normotensive (Wistar and Wistar-Kyoto (WKY)) and spontaneous hypertensive rats (SHR)
(2) to elucidate the mechanism(s) of action underling the relaxant effect in light of bioactive components. Methods: Vascular effects of HS aqueous fraction (AF) on isolated MA rings, as well as its mechanisms of action, were assessed using the contractility and intracellular microelectrode technique. The patch clamp technique was used to evaluate the effect of HS AF on the L-type calcium current. Extraction and enrichment of AF were carried out using liquid&ndash
liquid extraction, and the yield was analyzed using HPLC. Results: The HS AF induced a concentration-dependent relaxant effect on MA rings of SHR (EC50 = 0.83 ±
0.08 mg/mL), WKY (EC50 = 0.46 ±
0.04 mg/mL), and Wistar rats (EC50 = 0.44 ±
0.08 mg/mL) pre-contracted with phenylephrine (10 µ
M). In Wistar rats, the HS AF maximum relaxant effect was not modified after endothelium removal or when a guanylate cyclase inhibitor (ODQ, 10 µ
M) and a selective &beta
2-adrenergic receptor antagonist (ICI-118551, 1 µ
M) were incubated with the preparation. Otherwise, it was reduced by 34.57 ±
10.66% when vascular rings were pre-contracted with an 80 mM [K+] solution (p <
0.001), which suggests an effect on ionic channels. HS AF 2 mg/mL significantly decreased the peak of the L-type calcium current observed in cardiac myocytes by 24.4%. Moreover, though the vasorelaxant effect of HS, AF was reduced by 27% when the nonselective potassium channels blocker (tetraethylammonium (TEA) 20 mM) was added to the bath (p <
0.01). The extract did not induce a membrane hyperpolarization of smooth muscle cells, which might suggest an absence of a direct effect on background potassium current. Conclusion: These results highlight that the antihypertensive effect of HS probably involves a vasorelaxant effect on small resistance arteries, which is endothelium independent. L-type calcium current reduction contributes to this effect. The results could also provide a link between the vasorelaxant effect and the bioactive compounds, especially anthocyanins.
Databáze: OpenAIRE
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