Cloning and Heterologous Expression of Genes from the Kinamycin Biosynthetic Pathway of Streptomyces murayamaensis
| Autor: | Steven J. Gould, John R. Carney, Seong-Tshool Hong |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pharmacology
Genetics Streptomycetaceae Genes Fungal Restriction Mapping Carbazoles Gene Expression Kinamycin Molecular cloning Biology biology.organism_classification Streptomyces Anti-Bacterial Agents Polyketide genomic DNA Plasmid Multigene Family Drug Discovery Cosmid Vector Heterologous expression Cloning Molecular |
| Zdroj: | The Journal of Antibiotics. 51:50-57 |
| ISSN: | 1881-1469 0021-8820 |
| Popis: | The genes for most of the biosynthesis of the kinamycin antibiotics have been cloned and heterologously expressed. Genomic DNA of Streptomyces murayamaensis was partially digested with MboI and a library of approximately 40 kb fragments in E. coli XL1-BlueMR was prepared using the cosmid vector pOJ446. Hybridization with the actI probe from the actinorhodin polyketide synthase genes identified two clusters of polyketide genes. After transferal of these clusters to S. lividans ZX7, expression of one cluster was established by HPLC with photodiode array detection. Peaks were identified from the kin cluster for dehydrorabelomycin, kinobscurinone, and stealthin C, which are known intermediates in kinamycin biosynthesis. Two shunt metabolites, kinafluorenone and seongomycin were also identified. The structure of the latter was determined from a quantity obtained from large-scale fermentation of one of the clones. |
| Databáze: | OpenAIRE |
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