Immunophenotypic measurable residual disease (MRD) in acute myeloid leukemia: Is multicentric MRD assessment feasible?
| Autor: | Georgine E. de Greef, Nancy Boeckx, Bronno van der Holt, Vincent H.J. van der Velden, Rik A. Brooimans, Ngoc M. Van, Gerrit Jan Schuurhuis, Jeroen G. te Marvelde, Erik Huys, J. Slomp, Angèle Kelder, Frank Preijers, Antoinette Heijs |
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| Přispěvatelé: | Immunology, Medical Oncology, Hematology |
| Rok vydání: | 2019 |
| Předmět: |
Male
Oncology Cancer Research medicine.medical_specialty Neoplasm Residual Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] Concordance Disease Sensitivity and Specificity Immunophenotyping 03 medical and health sciences All institutes and research themes of the Radboud University Medical Center 0302 clinical medicine Internal medicine hemic and lymphatic diseases medicine Humans business.industry Myeloid leukemia Hematology Flow Cytometry Leukemia Myeloid Acute Multicenter study 030220 oncology & carcinogenesis Female business Biomarkers After treatment 030215 immunology |
| Zdroj: | Leukemia Research, 76, 39-47. Elsevier Ltd. Leukemia Research, 76, pp. 39-47 Leukemia Research, 76, 39-47 |
| ISSN: | 1873-5835 0145-2126 |
| Popis: | Flow-cytometric detection of now termed measurable residual disease (MRD) in acute myeloid leukemia (AML) has proven to have an independent prognostic impact. In a previous multicenter study we developed protocols to accurately define leukemia-associated immunophenotypes (LAIPs) at diagnosis. It has, however, not been demonstrated whether the use of the defined LAIPs in the same multicenter setting results in a high concordance between centers in MRD assessment. In the present paper we evaluated whether interpretation of list-mode data (LMD) files, obtained from MRD assessment of previously determined LAIPs during and after treatment, could reliably be performed in a multicenter setting. The percentage of MRD positive cells was simultaneously determined in totally 173 LMD files from 77 AML patients by six participating centers. The quantitative concordance between the six participating centers was meanly 84%, with slight variation of 75%-89%. In addition our data showed that the type and number of LAIPs were of influence on the performance outcome. The highest concordance was observed for LAIPs with cross-lineage expression, followed by LAIPs with an asynchronous antigen expression. Our results imply that immunophenotypic MRD assessment in AML will only be feasible when fully standardized methods are used for reliable multicenter assessment. ispartof: LEUKEMIA RESEARCH vol:76 pages:39-47 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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