Pyramidal and Stellate Cell Specificity of Grid and Border Representations in Layer 2 of Medial Entorhinal Cortex
| Autor: | Andrea Burgalossi, Robert K. Naumann, Michael Brecht, Christian L. Ebbesen, Helene Schmidt, Saikat Ray, Dominik Spicher, Qiusong Tang |
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| Jazyk: | angličtina |
| Předmět: |
Male
Cell type Neuroscience(all) Action Potentials Biology Calbindin Report Border cells medicine Extracellular Animals Entorhinal Cortex Theta Rhythm Neurons General Neuroscience Dentate gyrus Pyramidal Cells food and beverages Anatomy Entorhinal cortex Rats medicine.anatomical_structure Space Perception Hepatic stellate cell Pyramidal cell Neuroscience |
| Zdroj: | Neuron |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/j.neuron.2014.11.009 |
| Popis: | Summary In medial entorhinal cortex, layer 2 principal cells divide into pyramidal neurons (mostly calbindin positive) and dentate gyrus-projecting stellate cells (mostly calbindin negative). We juxtacellularly labeled layer 2 neurons in freely moving animals, but small sample size prevented establishing unequivocal structure-function relationships. We show, however, that spike locking to theta oscillations allows assigning unidentified extracellular recordings to pyramidal and stellate cells with ∼83% and ∼89% specificity, respectively. In pooled anatomically identified and theta-locking-assigned recordings, nonspatial discharges dominated, and weakly hexagonal spatial discharges and head-direction selectivity were observed in both cell types. Clear grid discharges were rare and mostly classified as pyramids (19%, 19/99 putative pyramids versus 3%, 3/94 putative stellates). Most border cells were classified as stellate (11%, 10/94 putative stellates versus 1%, 1/99 putative pyramids). Our data suggest weakly theta-locked stellate border cells provide spatial input to dentate gyrus, whereas strongly theta-locked grid discharges occur mainly in hexagonally arranged pyramidal cell patches and do not feed into dentate gyrus. Highlights • Stellates and pyramids can be putatively assigned by spike locking to theta • Grid cells in entorhinal layer 2 largely correspond to putative pyramidal cells • Border responses in layer 2 largely correspond to putative stellate cells Tang et al. investigated the cellular basis of spatial representations in layer 2 of medial entorhinal cortex and reported a cell-type specificity of spatial discharges: while most grid cells were putative pyramidal neurons, border cells were mainly putative stellate cells. |
| Databáze: | OpenAIRE |
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