Assessment of a 27-kDa antigen in enzyme-linked immunosorbent assay for the diagnosis of fasciolosis in Vietnamese patients
| Autor: | Nguyen Van De, Pierre Dorny, T. H. Le, T. G. T. Nguyen, Tinh Doan, Jozef Vercruysse, T. H. T. Dao |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Fasciola gigantica Feces Hepatic Zoonoses Diagnosis Asia Southeast Anthelmintics Fasciola biology Liver Diseases Enzyme-linked immunosorbent assay (ELISA) Helminthic diseases Middle Aged Hospitalization Infectious Diseases Triclabendazole Vietnam ELISA Female Antibody medicine.drug Adult Fascioliasis Adolescent Helminthiasis Antibodies Helminth Enzyme-Linked Immunosorbent Assay Assessment Sensitivity and Specificity Antibodies Diagnosis Differential Young Adult Antigen parasitic diseases medicine Fasciola hepatica Animals Humans Fasciolosis Antigens Aged Public Health Environmental and Occupational Health biology.organism_classification medicine.disease Antigens Helminth Immunology biology.protein Parasitology Benzimidazoles |
| Zdroj: | Tropical medicineinternational health : TMIH. 15(4) |
| ISSN: | 1365-3156 |
| Popis: | Summary Fasciolosis has emerged as an important zoonotic disease in many parts of the world. In recent years, an increasing number of human cases were reported in Vietnam. In this study, the 27-kDa component protein from the excretory/secretory production of adult Fasciola gigantica, purified by high performance liquid chromatography, was assessed in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against Fasciola spp. for diagnosis of human fasciolosis. The ELISA showed a high sensitivity (100%) and specificity (97.67%) when tested on patients with fasciolosis, other parasitic infections, cholangiocarcinoma and on healthy controls. The assay was applied for diagnosis on 143 patients in the Viet Duc-Hanoi hospital who presented with clinical signs of liver disease and lesions in their livers as shown by imaging techniques. Antibodies were found in 37 (25.9%) of these patients, of whom only 3 shed Fasciola eggs in their stools (2.1%). The excellent response to triclabendazole treatment of 37 sero-positive patients confirmed the diagnosis of fasciolosis. This study demonstrated the diagnostic potential for human fasciolosis of the 27-kDa antigen ELISA. Fasciolosis should be considered in the differential diagnosis of hepatic disease in Vietnam. |
| Databáze: | OpenAIRE |
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