Improved detection of human pancreatic proteins separated by two-dimensional isoelectric focusing/sodium dodecyl sulphate gel electrophoresis using a combined staining procedure
| Autor: | G. Appelt, R. Rogos, Marion Hänsler |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Gel electrophoresis
Two-dimensional gel electrophoresis Chromatography Staining and Labeling Isoelectric focusing Hydrolases Coomassie Brilliant Blue Proteins General Chemistry Gel electrophoresis of proteins Staining Silver stain chemistry.chemical_compound chemistry Pancreatic Juice Humans Electrophoresis Gel Two-Dimensional Electrophoresis Polyacrylamide Gel Isoelectric Focusing Densitometry Glycoproteins |
| Zdroj: | Journal of chromatography. 563(1) |
| Popis: | In order to assess secretory pancreatic proteins in a two-dimensional isoelectric focusing/sodium dodecyl sulphate electrophoresis gel, a highly sensitive double-staining method with Coomassie Brilliant Blue followed by silver stain was used. This combined procedure afforded more distinct spots and additional bands, particularly glycoproteins, than either silver or Coomassie Blue staining alone. As measurements of dye volumes by densitometry have shown, double staining of two-dimensional separated pancreatic proteins is up to twenty times more sensitive than the usual Coomassie Brilliant Blue staining. |
| Databáze: | OpenAIRE |
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