Studies on the action and chemical nature of the nuclear factor promoting degradation of chromatin DNA
| Autor: | Tumanishvili Gd, Dzidziguri Dv, K.M. Jandieri, N.D. Mgvdeladze, N.G. Avalishvili |
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| Rok vydání: | 1974 |
| Předmět: |
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Biology In Vitro Techniques Kidney chemistry.chemical_compound Ribonucleases medicine Animals Trypsin Cell Nucleus Deoxyribonucleases Pancreatic DNase Tissue Extracts RNA Cell Biology DNA Hydrogen-Ion Concentration Molecular biology Chromatin Cell biology Rats medicine.anatomical_structure Nucleoproteins chemistry Liver Cytoplasm Degradation (geology) DNase I hypersensitive site |
| Zdroj: | Experimental cell research. 84(1) |
| ISSN: | 0014-4827 |
| Popis: | Chromatin DNA of liver and kidney, obtained by the method of Dingman & Sporn, is inaccessible in 0.14 M NaCl to pancreatic DNase and cytoplasmic DNase. Under the combined action of DNase and nuclear extract (NE) (extraction with 0.14 M NaCl) on chromatin, the DNA of the latter is intensively degraded. The action of NE is tissue-specific—liver NE has almost no effect on kidney chromatin DNA degradation. The removal of protein or RNA from NE deprives it of its ability to accelerate chromatin DNA degradation by DNases. It is assumed that the active part of NE is a complex of a protein and RNA. Here, tissue specificity is determined by both components of this complex. The biological role of the nuclear factor promoting chromatin DNA degradation is not known at present. |
| Databáze: | OpenAIRE |
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