Upstream Stimulatory Factor Regulates Constitutive Expression and Hormonal Suppression of the 90K (Mac-2BP) Protein
| Autor: | Giorgio Napolitano, Hyun-Kyung Chung, Cesidio Giuliani, Dinah S. Singer, Leonard D. Kohn, Antonino Grassadonia, Minoru Nakazato, Nicola Tinari, T. Kevin Howcroft, Bruno Fiorentino, Stefano Iacobelli |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
5' Flanking Region
Recombinant Fusion Proteins Molecular Sequence Data USF2 Response element USF1 Thyrotropin Electrophoretic Mobility Shift Assay Response Elements Transfection Upstream Stimulatory Factor Cell Line Interferon-gamma Endocrinology Interferon Sequence Homology Nucleic Acid Gene expression Cyclic AMP medicine Animals Insulin Insulin-Like Growth Factor I Binding site Luciferases Promoter Regions Genetic Extracellular Matrix Proteins Binding Sites Base Sequence biology Proteins Sequence Analysis DNA Molecular biology Rats Gene Expression Regulation biology.protein Upstream Stimulatory Factors Carrier Proteins Protein Binding medicine.drug |
| Zdroj: | Endocrinology. 148:3507-3517 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2007-0024 |
| Popis: | We previously reported that hormones important for the normal growth and function of FRTL-5 rat thyroid cells, TSH, or its cAMP signal plus insulin or IGF-I, could transcriptionally suppress constitutive and γ-interferon (IFN)-increased synthesis of the 90K protein (also known as Mac-2BP). Here we cloned the 5′-flanking region of the rat 90K gene and identified a minimal promoter containing an interferon response element and a consensus E-box or upstream stimulator factor (USF) binding site, which are highly conserved in both the human and murine genes. We show that suppression of constitutive and γ-IFN-increased 90K gene expression by TSH/cAMP plus insulin/IGF-I depends on the ability of the hormones to decrease the binding of USF to the E-box, located upstream of the interferon response element. This site is required for the constitutive expression of the 90K gene. Transfection with USF1 and USF2 cDNAs increases constitutive promoter activity, attenuates the ability of TSH/cAMP plus insulin/IGF-I to decrease constitutive or γ-IFN-increased 90K gene expression but does not abrogate the ability of γ-IFN itself to increase 90K gene expression. |
| Databáze: | OpenAIRE |
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