Assay method for antitumor l-methionine γ-lyase: comprehensive kinetic analysis of the complex reaction with l-methionine
| Autor: | Kenji Mitsushima, Hidehiko Tanaka, Kenji Inagaki, Kenji Soda, Akio Takimoto, Nobuyoshi Esaki, Shigeo Yagi, Tomoaki Takakura |
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| Rok vydání: | 2004 |
| Předmět: |
Antimetabolites
Antineoplastic Stereochemistry Kinetics Biophysics Methanethiol Biochemistry Carcinoma Lewis Lung chemistry.chemical_compound Methionine Animals Enzyme kinetics Molecular Biology Pyridoxal chemistry.chemical_classification biology Cell Biology Proteus Lyase Combinatorial chemistry Recombinant Proteins Enzyme assay Butyrates Carbon-Sulfur Lyases Enzyme chemistry biology.protein |
| Zdroj: | Analytical Biochemistry. 327:233-240 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2004.01.024 |
| Popis: | L-Methionine gamma-lyase (EC 4.4.1.11) is a pyridoxal 5'-phosphate-dependent multifunctional enzyme. Measuring the initial velocity of alpha-ketobutyrate production by alpha,gamma-elimination of L-methionine catalyzed by L-methionine gamma-lyase is not very feasible, because the enzyme simultaneously catalyzes both gamma-replacement and alpha,gamma-elimination. To develop an accurate enzyme assay, the comprehensive enzyme kinetics needed to be elucidated by progress curve analysis on the basis of a reaction model for conversion of L-methionine to alpha-ketobutyrate, methanethiol, and ammonia with pyridoxal 5'-phosphate as a cofactor. Kinetic parameters were determined by linear transformation using an approximation of a Maclaurin series from the whole velocity of alpha-ketobutyrate production including alpha,gamma-elimination and gamma-replacement. The significance of gamma-replacement was revealed both theoretically and practically by the kinetic analysis. The enzyme activity was standardized and represented as the Vmax value taking into consideration gamma-replacement in the presence of L-methionine at 37 degrees C and pH 8.0. The novel method that we proposed is accurate, sensitive, reproducible, and linear over a wide range for the determination of L-methionine gamma-lyase activity. |
| Databáze: | OpenAIRE |
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