OLA-Simple: A software-guided HIV-1 drug resistance test for low-resource laboratories
| Autor: | Justin D. Vrana, Parker S. Ruth, Ute Dagmar Feucht, Laddawan Laomanit, Ingrid A. Beck, Annie Wong-On-Wing, Daisy Ko, David McIntyre, Jonathan Lim, Enos Kline, Ross S. Milne, Barry R. Lutz, James J. Lai, Eric Klavins, Nicole Ngo-Giang-Huong, Ruth Kanthula, Nuttada Panpradist, Isaac So, Lisa M. Frenkel, Michael H. Chung, Nikki Higa, Theresa M. Rossouw, Jaime Soria, Gonzague Jourdain |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
NNRTI medicine.medical_specialty Research paper Genotype Genotyping Techniques NRT Regimen switching Low resource Anti-HIV Agents Resistance Human immunodeficiency virus (HIV) lcsh:Medicine HIV Infections Drug resistance Microbial Sensitivity Tests medicine.disease_cause General Biochemistry Genetics and Molecular Biology Workflow 03 medical and health sciences Instructional software symbols.namesake 0302 clinical medicine Software Drug Resistance Viral medicine Humans Medical physics Genotyping Sanger sequencing lcsh:R5-920 business.industry HIV genotyping assay lcsh:R Computational Biology General Medicine 3. Good health 030104 developmental biology Research Design 030220 oncology & carcinogenesis Mutation symbols HIV-1 Reagent Kits Diagnostic business lcsh:Medicine (General) HIV drug resistance |
| Zdroj: | EBioMedicine, Vol 50, Iss, Pp 34-44 (2019) EBioMedicine |
| ISSN: | 2352-3964 |
| Popis: | Background: HIV drug resistance (HIVDR) testing can assist clinicians in selecting treatments. However, high complexity and cost of genotyping assays limit routine testing in settings where HIVDR prevalence has reached high levels. Methods: The oligonucleotide ligation assay (OLA)-Simple kit was developed for detection of HIVDR against first-line non-nucleoside/nucleoside reverse transcriptase inhibitors and validated on 672 codons (168 specimens) from subtypes A, B, C, D, and AE. The kit uses dry reagents to facilitate assay setup, lateral flow devices for visual HIVDR detections, and in-house software with an interface for guiding users and analyzing results. Findings: HIVDR analysis of specimens by OLA-Simple compared to Sanger sequencing revealed 99.6 ± 0.3% specificity and 98.2 ± 0.9% sensitivity, and compared to high-sensitivity assays, 99.6 ± 0.6% specificity and 86.2 ± 2.5% sensitivity, with 2.6 ± 0.9% indeterminate results. OLA-Simple was performed more rapidly compared to Sanger sequencing ( |
| Databáze: | OpenAIRE |
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