Multiple growth factors are released from mechanically injured vascular smooth muscle cells
| Autor: | L. D. Horwitz, Dilsher Nawaz, C. J. Ray, S. T. Crowley, R. A. Majack |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Vascular smooth muscle Physiology medicine.medical_treatment Basic fibroblast growth factor Immunoblotting Fibroblast growth factor Antibodies Muscle Smooth Vascular chemistry.chemical_compound Epidermal growth factor Physiology (medical) Internal medicine medicine Animals Cells Cultured Platelet-Derived Growth Factor biology Epidermal Growth Factor business.industry Growth factor Endocrinology chemistry Culture Media Conditioned Immunology biology.protein Cattle Fibroblast Growth Factor 2 Mitogens Cardiology and Cardiovascular Medicine business Platelet-derived growth factor receptor Transforming growth factor |
| Zdroj: | The American journal of physiology. 269(5 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | Local release of mitogenic and chemotactic signals during angioplasty-induced vascular injury may initiate restenosis. We investigated whether mechanical injury to vascular smooth muscle cells (VSMC) results in the release of biologically active peptide growth factors. Monolayers of bovine SMC cultures were mechanically injured by cell scraping. Conditioned medium (CM) from control and injured SMC cultures was collected, and the mitogenic activity was measured by [3H]thymidine incorporation in recipient SMC cultures. Mitogenic activity from injured CM was detected within 15 min after injury. When the CM from injured cells was removed 15 min after injury and replaced with serum-free media, there was no detectable mitogenic activity in the replacement CM assessed 1-6 days postinjury. Suramin, a nonspecific peptide growth factor antagonist, significantly inhibited the mitogenic activity of injured CM. Basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF A chain), and epidermal growth factor (EGF) were detected in CM from injured cells by immunoblot analysis. The mitogenic activity of injured CM was significantly inhibited with neutralizing antibodies to bFGF (34%), PDGF-AA (32%), PDGF-BB (25%), and EGF (25%). A neutralizing antibody to transforming growth factor (TGF)-beta had no effect. In conclusion, bFGF, PDGF, and EGF are immediately released from mechanically injured VSMC. VSMC likely contain preformed, biologically active growth factors that are efficiently released from the cell cytoplasm following mechanical injury. Conditioned medium from injured VSMC is highly mitogenic, and this activity is probably due to multiple growth factors interacting synergistically. |
| Databáze: | OpenAIRE |
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