Analysis of variation in virulence of Beauveria bassiana against insect pests of pigeonpea using qPCR
| Autor: | G. Senthilraja, Subbarayalu Mohankumar, Thiruvengadam Raguchander, T. Anand, Ramasamy Samiyappan |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Virulence Factors media_common.quotation_subject Beauveria bassiana Virulence Insect Bassiana Helicoverpa armigera Real-Time Polymerase Chain Reaction 01 natural sciences Applied Microbiology and Biotechnology Virulence factor 03 medical and health sciences Cajanus Animals Beauveria media_common biology Chitinases General Medicine biology.organism_classification Culture Media Lepidoptera 010602 entomology 030104 developmental biology Biological Variation Population Maruca vitrata Chitinase biology.protein |
| Zdroj: | Journal of Basic Microbiology. 58:277-282 |
| ISSN: | 0233-111X |
| Popis: | Beauveria bassiana is a broad spectrum microbial bioagent used for the control of agriculturally important insect pests. However, in our experiments, two virulent isolates of B. bassiana (B2 and B10) showed specific preference toward Maruca vitrata and Helicoverpa armigera of pigeonpea. To better understand this feature, we developed a qPCR assay to quantify the chitinase (virulence factor) of B. bassiana during the infection process. Isolates of B. bassiana were grown on insect cuticle amended medium and minimal medium (without insect cuticle) to assess the induction of chitinase. Our results revealed a positive correlation between expression of chitinase by B. bassiana and the substrates (with or without cuticles of M. vitrata and H. armigera) used. This study showcases the methodology to quantify the chitinase and analysis of variation in virulence of B. bassiana (B2 and B10) against M. vitrata and H. armigera. |
| Databáze: | OpenAIRE |
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