Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage
| Autor: | Christopher P. Saint, Andrew Michelmore, Nekane Reta, Nicolas H. Voelcker, Beatriz Prieto-Simón |
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| Přispěvatelé: | Reta, Nekane, Michelmore, Andrew P, Saint, Christopher P, Prieto, Simon Beatriz, Voelcker, Nico H |
| Rok vydání: | 2015 |
| Předmět: |
Analyte
Materials science viruses Biomedical Engineering Biophysics Nanotechnology 02 engineering and technology Biosensing Techniques 010402 general chemistry Porous silicon label free 01 natural sciences Buffer (optical fiber) electrochemical immunoassay Bacteriophage MS2 Electrochemistry Levivirus Detection limit Immunoassay biology nanochannel blockage MS2 bacteriophage General Medicine Electrochemical Techniques equipment and supplies 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences porous silicon Membrane Electrode Differential pulse voltammetry 0210 nano-technology Antibodies Immobilized Porosity Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 80 |
| ISSN: | 1873-4235 |
| Popis: | A proof of concept for the label-free detection of bacteriophage MS2, a model indicator of microbiological contamination, is validated in this work as a porous silicon (pSi) membrane-based electrochemical biosensor. PSi membranes were used to afford nanochannel architectures. The sensing mechanism was based on the nanochannel blockage caused by MS2 binding to immobilized capture antibodies. This blockage was quantified by measuring the oxidation current of the electroactive species reaching the electrode surface, by means of differential pulse voltammetry (DPV). The immunosensor showed a limit of detection of 6 pfu/mL in buffer, allowing the detection of MS2 to levels commonly found in real-world applications, and proved to be unaffected by matrix effects when analyzing MS2 in reservoir water. This platform enables the straightforward, direct and sensitive detection of a broad range of target analytes and constitutes a promising approach towards the development of portable electronic point of sample analysis devices. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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