Phylogenetic relationships of nonaxenic filamentous cyanobacterial strains based on 16S rRNA sequence analysis
| Autor: | Bart Nelissen, Annick Wilmotte, Rupert De Wachter, Raymond De Baere |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cyanobacteria
biology Phylogenetic tree Base Sequence Molecular Sequence Data Ribosomal RNA biology.organism_classification 16S ribosomal RNA Polymerase Chain Reaction Evolution Molecular RNA Bacterial Phylogenetics RNA Ribosomal 16S Botany Genetics RRNA Operon Axenic Oligomer restriction Oligonucleotide Probes Molecular Biology Ecology Evolution Behavior and Systematics Phylogeny |
| Zdroj: | Journal of molecular evolution |
| ISSN: | 0022-2844 |
| Popis: | In order to determine the nearly complete 16S rRNA gene sequences of cyanobacteria originating from nonaxenic cultures, a cyanobacterium-specific oligonucleotide probe was developed to distinguish polymerase chain reaction (PCR) products of the cyanobacterial rRNA operons from those resulting from amplification of contaminating bacteria. Using this screening method the 16S rRNA genes of four nonaxenic filamentous cyanobacterial strains belonging to the genera Leptolyngbya and Oscillatoria were cloned and sequenced. For the genus Leptolyngbya, the 16S rRNA sequence of the axenic strain PCC 73110 was also determined. Phylogenetic trees were constructed based on complete and partial sequences. The results show that the strains Leptolyngbya foveolarum Komárek 1964/112, Leptolyngbya sp. VRUC 135 Albertano 1985/1, and Leptolyngbya boryanum PCC 73110 belong to the same cluster. Strain Oscillatoria cf. corallinae SAG 8.92, which contains the rare photosynthetic pigment CU-phycoerythrin, is not closely related to other CU-phycoerythrin-containing cyanobacteria. Oscillatoria agardhii CYA 18, which is a representative of planktonic Oscillatoria species that form toxic blooms in Norwegian inland waters, has no close relatives in the tree. |
| Databáze: | OpenAIRE |
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