A plaque technique for the titration of yellow fever virus and antisera
| Autor: | J.S. Porterfield |
|---|---|
| Rok vydání: | 1959 |
| Předmět: |
Antiserum
Tris food.ingredient Immune Sera Yellow fever Public Health Environmental and Occupational Health General Medicine Biology medicine.disease Molecular biology Virus chemistry.chemical_compound Infectious Diseases food chemistry Yellow Fever medicine biology.protein Humans Agar Parasitology Titration Yellow fever virus Antibody Encephalitis |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 53:458-466 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/0035-9203(59)90021-5 |
| Popis: | 1. 1) Yellow fever virus may be titrated by a plaque technique in cultures of chick embryo fibroblasts. The cells are grown and maintained under agar in a medium buffered with Tris (hydroxymethyl) aminomethane, using a conventional laboratory incubator without added carbon dioxide. 2. 2) The sensitivity of the technique approaches that of the mouse intracerebral test and exceeds that test in precision. 3. 3) Yellow fever neutralizing antibodies may be detected by a plaque inhibition test. 4. 4) The technique may be applied to a number of other viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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