Characterization of lactoferrin binding to the MAC-T bovine mammary epithelial cell line using a biotin-avidin technique
| Autor: | John J. Rejman, J. D. Turner, Stephen P. Oliver |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cell
Biotin Biochemistry Epithelium Cell Line chemistry.chemical_compound Mammary Glands Animal medicine Animals Binding site chemistry.chemical_classification biology Cell growth Lactoferrin Cell Membrane Epithelial Cells Avidin Immunohistochemistry Molecular biology medicine.anatomical_structure chemistry Transferrin biology.protein Cattle Female Cell Division Protein Binding |
| Zdroj: | International Journal of Biochemistry. 26:201-206 |
| ISSN: | 0020-711X |
| DOI: | 10.1016/0020-711x(94)90146-5 |
| Popis: | 1. 1. A non-radioisotopic method utilizing a biotin-avidin approach was used to characterize lactoferrin binding to the clonal MAC-T bovine mammary epithelial cell line. 2. 2. Binding of lactoferrin to MAC-T cells and isolated membranes was specific and saturable. 3. 3. Unlabeled lactoferrin competed for and displaced biotin-labeled lactoferrin from binding sites on mammary epithelial cells. In contrast, unlabeled transferrin did not compete. 4. 4. Scatchard analysis of lactoferrin binding to MAC-T cell crude membranes was nonlinear, revealing two classes of binding sites with association constants ( K a ) of 2.36 × 10 7 and 3.36 × 10 6 M −1 . 5. 5. Binding of lactoferrin to MAC-T cells may be associated with the initial events which result in decreased MAC-T cell proliferation. |
| Databáze: | OpenAIRE |
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