A novel small animal model for HIV-1 infection
| Autor: | Smadar Even Tov Friedman, Mila Ayash-Rashkovsky, Fabian D. Arditti, Gadi Borkow, Zvi Bentwich, Yair Reisner |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Human immunodeficiency virus (HIV)
CD4-CD8 Ratio Biology HIV Antibodies medicine.disease_cause Lymphocyte Activation Biochemistry Peripheral blood mononuclear cell Interferon-gamma Mice Immune system Small animal Genetics medicine Animals Humans Molecular Biology Acquired Immunodeficiency Syndrome Mice Inbred BALB C Increased T cell activation HLA-DR Antigens Virology CD4 Lymphocyte Count Disease Models Animal medicine.anatomical_structure Immunology HIV-1 Leukocytes Mononuclear Bone marrow CD8 Biotechnology T-Lymphocytes Cytotoxic |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 19(9) |
| ISSN: | 1530-6860 |
| Popis: | Lethally irradiated normal BALB/c mice, reconstituted with murine SCID bone marrow and engrafted with human PBMC (Trimera mice), were used to establish a novel murine model for HIV-1 infection. The Trimera mice were successfully infected with different clades and primary isolates of T- and M-tropic HIV-1, with the infection persisting in the animals for 4-6 wk. Rapid loss of the human CD4+ T cells, decrease in CD4/CD8 ratio, and increased T cell activation accompanied the viral infection. All HIV-1 infected animals were able to generate both primary and secondary immune responses, including HIV specific human humoral and cellular responses. In addition to testing the efficacy of new antiviral compounds, this new murine HIV-1 model may be used for studying host-virus interactions and, most importantly, for screening and developing potential HIV-1 protective vaccines and adjuvants (Ayash-Rashkovsky et al., http://www.fasebj.org/cgi/doi/10.1096/fj.04-3185fje; doi:10.1096/fj.04-3185fje.). |
| Databáze: | OpenAIRE |
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