Salmonella chitosan nanoparticle vaccine administration is protective against Salmonella Enteritidis in broiler birds

Autor: Keila Y. Acevedo-Villanueva, Ramesh K Selvaraj, Revathi Shanmugasundaram, Renukaradhy J. Gourapura, Gabriel Akerele, Sankar Renu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Bacterial Diseases
Salmonella
Pathology and Laboratory Medicine
medicine.disease_cause
Biochemistry
Poultry
Medical Conditions
Microbial Physiology
Medicine and Health Sciences
Public and Occupational Health
Bacterial Physiology
Booster Doses
Immune Response
Vaccines
Multidisciplinary
Attenuated vaccine
Eukaryota
Salmonella vaccine
Vaccination and Immunization
Bacterial Pathogens
Infectious Diseases
Medical Microbiology
Vertebrates
Medicine
Pathogens
Research Article
Infectious Disease Control
Salmonella Vaccines
Salmonella enteritidis
Science
Immunology
Biology
Vaccines
Attenuated
Microbiology
Birds
Enterobacteriaceae
medicine
Splenocyte
Animals
Microbial Pathogens
Chitosan
Bacteria
Organisms
Broiler
Biology and Life Sciences
Proteins
Bacteriology
Amniotes
Humoral immunity
Preventive Medicine
Flock
Zoology
Chickens
Flagellin
Zdroj: PLoS ONE, Vol 16, Iss 11, p e0259334 (2021)
PLoS ONE, Vol 16, Iss 11 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Salmonella control strategies include vaccines that help reduce the spread of Salmonella in poultry flocks. In this study we evaluated the efficacy of administering a live Salmonella vaccine followed by a killed Salmonella chitosan nanoparticle (CNP) vaccine booster on the cellular and humoral immunity of broilers. The CNP vaccine was synthesized with Salmonella Enteritidis (S. Enteritidis) outer-membrane-proteins (OMPs) and flagellin-proteins. At d1-of-age, one-hundred-sixty-eight chicks were allocated into treatments: 1) No vaccine, 2) Live vaccine (Poulvac®ST), 3) CNP vaccine, 4) Live+CNP vaccine. At d1-of-age, birds were orally vaccinated with PBS, Live vaccine, or CNP. At d7-of-age, the No vaccine, Live vaccine and CNP vaccine groups were boosted with PBS and the Live+CNP vaccine group was boosted with CNP. At d14-of-age, birds were challenged with 1×109 CFU/bird S. Enteritidis. There were no significant differences in body-weight-gain (BWG) or feed-conversion-ratio (FCR). At 8h-post-challenge, CNP and Live+CNP-vaccinated birds had 17% and 24% greater levels (PSalmonella OMPs IgA in bile, respectively, compared to control. At d28-of-age, CNP, Live, and Live+CNP-vaccinated birds had 33%, 18%, and 24% greater levels (PSalmonella OMPs IgA in bile, respectively, compared to control. At d14-of-age, Live+CNP-vaccinated birds had 46% greater levels (PSalmonella OMPs IgY in serum, compared to control. At d21-of-age, splenocytes from CNP and Live-vaccinated birds had increased (PS. Enteritidis cecal loads (PS. Enteritidis in broilers.
Databáze: OpenAIRE
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