Analysis on the transcriptome information of two different wheat mutants and identification of salt-induced differential genes
| Autor: | Peihan Gu, Wenji Liang, Shaoxiong Wang, Yinzhu Shen, Xiaoli Ma, Xiaoli Jin, Zhanjing Huang, Yuxin Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene Biophysics RNA-Seq Sodium Chloride 01 natural sciences Biochemistry Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Stress Physiological Arabidopsis thaliana KEGG Molecular Biology Gene Triticum Plant Proteins Genetics biology food and beverages RNA Salt-Tolerant Plants Cell Biology biology.organism_classification 030104 developmental biology Mutation DNA microarray 010606 plant biology & botany |
| Zdroj: | Biochemical and Biophysical Research Communications. 473:1197-1204 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2016.04.039 |
| Popis: | This study established a wheat transcriptome library using RH8706-49 and RH8706-34. Salt-induced differential genes were screened by Illumina RNA sequencing (RNA-Seq). Five differential genes were chosen to study the functions by combining transcript sequencing result and gene chip. The expression changes of these five differential genes were analyzed using real-time quantitative PCR (qRT-PCR) technique to determine the reliability and accuracy of transcriptome sequencing and transplanted into Arabidopsis thaliana to obtain transgenic homozygote plants for the salt tolerance test. The salt tolerance test results show that the transgenic plants grew far better than the wild-type plant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect