Expression of platelet-derived growth factor B-chain in neointimal smooth muscle cells of balloon injured rabbit femoral arteries
| Autor: | Masakiyo Sasahara, F. Hazama, Masahiko Kinoshita, Kazunori Uchida, Naoki Morigami |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Neointima Pathology medicine.medical_specialty Time Factors Platelet-derived growth factor In situ hybridization Femoral artery Muscle Smooth Vascular chemistry.chemical_compound Restenosis medicine.artery medicine Animals Humans Receptors Platelet-Derived Growth Factor RNA Messenger Northern blot In Situ Hybridization Platelet-Derived Growth Factor Lagomorpha biology biology.organism_classification medicine.disease Femoral Artery Gene Expression Regulation chemistry biology.protein Rabbits Cardiology and Cardiovascular Medicine Angioplasty Balloon Cell Division Platelet-derived growth factor receptor |
| Zdroj: | Atherosclerosis. 124:9-23 |
| ISSN: | 0021-9150 |
| DOI: | 10.1016/0021-9150(95)05742-0 |
| Popis: | In order to obtain information about the developmental mechanisms of restenosis after angioplasty, we investigated an association between the expression of platelet-derived growth factors (PDGFs) and neointimal cell accumulation in rabbit femoral arteries subjected to balloon angioplasty. Northern analysis demonstrated that mRNA expression of PDGF B-chain (PDGF-B) increased markedly in the injured arteries, peaking at day 7 (sevenfold), and the transcripts remained augmented until day 21. Also transcripts of PDGF beta-receptor (PDGFR-beta) and alpha-receptor increased by 3- and 2.5-fold, respectively, but those of PDGF A-chain showed only a slight increase (1.5-fold). In situ hybridization and immunohistochemistry demonstrated the concordant expression of mRNA and protein for PDGF-B in the smooth muscle cells (SMCs) of injured vessels throughout the experiment. PDGF-B expression peaked in neointimal SMCs at day 7. In accordance with PDGF-B expression, cellular proliferation in neointima peaked at day 7, being followed by a dramatic increase of neointimal areas thereafter. Further, we demonstrated PDGFR-beta immunoreactivity in these neointimal cells with PDGF-B expression. Our data provide evidence that PDGF-B may stimulate vascular SMC proliferation and contribute to neointimal formation after angioplasty. |
| Databáze: | OpenAIRE |
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