Flavonoids Modulate the Proliferation of Neospora caninum in Glial Cell Primary Cultures
| Autor: | Alexandre M. Pinheiro, Ramon dos Santos El-Bachá, Cátia Suse de Oliveira Ribeiro, Bruno P.S. Pitanga, Suzana Braga-de-Souza, Silvia Lima Costa, Erica Etelvina Viana de Jesus, Victor Diogenes Amaral da Silva, Maria de Fátima Dias Costa, Rosan Barbosa de Matos |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
glia
Cell Neospora caninum Microbiology chemistry.chemical_compound Neospora parasitic diseases medicine Animals Immunologic Factors heterocyclic compounds flavonoid Rats Wistar nitric oxide (NO) Cells Cultured Flavonoids biology fungi food and beverages biology.organism_classification Virology Infectious Diseases medicine.anatomical_structure chemistry Gliosis Cell culture Sarcocystidae Neuroglia Parasitology Original Article medicine.symptom Luteolin |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 0023-4001 |
| Popis: | Neospora caninum (Apicomplexa; Sarcocystidae) is a protozoan that causes abortion in cattle, horses, sheep, and dogs as well as neurological and dermatological diseases in dogs. In the central nervous system of dogs infected with N. caninum, cysts were detected that exhibited gliosis and meningitis. Flavonoids are polyphenolic compounds that exhibit antibacterial, antiparasitic, antifungal, and antiviral properties. In this study, we investigated the effects of flavonoids in a well-established in vitro model of N. caninum infection in glial cell cultures. Glial cells were treated individually with 10 different flavonoids, and a subset of cultures was also infected with the NC-1 strain of N. caninum. All of the flavonoids tested induced an increase in the metabolism of glial cells and many of them increased nitrite levels in cultures infected with NC-1 compared to controls and uninfected cultures. Among the flavonoids tested, 3',4'-dihydroxyflavone, 3',4',5,7-tetrahydroxyflavone (luteolin), and 3,3',4',5,6-pentahydroxyflavone (quercetin), also inhibited parasitophorous vacuole formation. Taken together, our findings show that flavonoids modulate glial cell responses, increase NO secretion, and interfere with N. caninum infection and proliferation. |
| Databáze: | OpenAIRE |
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