The Actin-Based Motor Myosin Vb Is Crucial to Maintain Epidermal Barrier Integrity
| Autor: | Guy Serre, Marie Reynier, Alain Moga, Corinne Leprince, Sophie Allart, Dominique Goudounèche, Michel Simon |
|---|---|
| Přispěvatelé: | Unité différenciation épidermique et auto-immunité rhumatoïde (UDEAR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Physiopathologie Toulouse Purpan (CPTP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Microscopie Électronique Appliquée à la Biologie (CMEAB), Hôpital de Rangueil, CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), SYNELVIA, Toulouse Réseau Imagerie-Genotoul ( TRI-Genotoul), CCSD, Accord Elsevier |
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
0301 basic medicine Lamellar granule Biochemistry Cell junction 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Claudin-1 Myosin MESH: Tight Junctions / metabolism RNA Small Interfering MESH: Myosin Heavy Chains / genetics Cytoskeleton Cells Cultured MESH: Keratinocytes / metabolism integumentary system Chemistry MESH: Epidermis / pathology Cell biology Actin Cytoskeleton Protein Transport MESH: Intercellular Signaling Peptides and Proteins / metabolism medicine.anatomical_structure 030220 oncology & carcinogenesis [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases symbols Intercellular Signaling Peptides and Proteins MESH: Myosin Heavy Chains / metabolism Protein Binding MESH: Cells Cultured MESH: Actin Cytoskeleton / metabolism MESH: Protein Transport Myosin Type V Dermatology MESH: Myosin Type V / genetics Tight Junctions MESH: Actins / metabolism 03 medical and health sciences symbols.namesake MESH: Keratinocytes / pathology medicine Stratum corneum Humans MESH: Protein Binding MESH: Claudin-1 / metabolism Molecular Biology Actin MESH: Humans Myosin Heavy Chains MESH: rab GTP-Binding Proteins / metabolism MESH: RNA Small Interfering / genetics MESH: Myosin Type V / metabolism Cell Biology Golgi apparatus Actins MESH: Epidermis / metabolism 030104 developmental biology rab GTP-Binding Proteins Epidermis |
| Zdroj: | Journal of Investigative Dermatology Journal of Investigative Dermatology, Nature Publishing Group, 2019, 139 (7), pp.1430-1438. ⟨10.1016/j.jid.2018.12.021⟩ Journal of Investigative Dermatology, 2019, 139 (7), pp.1430-1438. ⟨10.1016/j.jid.2018.12.021⟩ |
| ISSN: | 0022-202X 1523-1747 |
| DOI: | 10.1016/j.jid.2018.12.021 |
| Popis: | International audience; Myosin Vb (Myo5b) is an unconventional myosin involved in the actin-dependent transport and tethering of intracellular organelles. In the epidermis, granular keratinocytes accumulate cytoplasmic lamellar bodies (LBs), secretory vesicles released at the junction with the stratum corneum that participate actively in the maintenance of the epidermal barrier. We have previously demonstrated that LB biogenesis is controlled by the Rab11a guanosine triphosphate hydrolase, known for its ability to recruit the Myo5b motor. In order to better characterize the molecular pathway that controls LB trafficking, we analyzed the role of F-actin and Myo5b in the epidermis. We demonstrated that LB distribution in granular keratinocytes was dependent on a dynamic F-actin cytoskeleton. Myo5b was shown to be highly expressed in granular keratinocytes and associated with corneodesmosin-loaded LB. In reconstructed human epidermis, Myo5b silencing led to epidermal barrier defects associated with structural alterations of the stratum corneum and a reduced pool of LB showing signs of disordered maturation. Myo5b depletion also disturbed the expression and distribution of both LB cargoes and junctional components, such as claudin-1, which demonstrates its action on both LB trafficking and junctional complex composition. Together, our data reveal the essential role of Myo5b in maintaining the epidermal barrier integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|