Anti-β1Integrin IgG Inhibits Pulmonary Macrometastasis and the Size of Micrometastases from a Murine Mammary Carcinoma
| Autor: | Hugh F. Pross, Bruce E. Elliott, Kristofer Rubin, Audrey Niemann, Peter Ekblom |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Integrins
Lung Neoplasms Cell Adhesion Molecules Neuronal Integrin Metastasis Mice Breast cancer Immunity In vivo Cell Adhesion medicine Animals Macrometastasis Extracellular Matrix Proteins Lung biology business.industry Integrin beta1 Mammary Neoplasms Experimental Tenascin General Medicine medicine.disease Fibronectins medicine.anatomical_structure Immunoglobulin G Immunology Mice Inbred CBA biology.protein Cancer research Female Laminin Antibody business Cell Division |
| Zdroj: | Cell Adhesion and Communication. 1:319-332 |
| ISSN: | 1029-2314 |
| DOI: | 10.3109/15419069409097263 |
| Popis: | In the present report, we investigated the possible importance of beta 1 integrins in the growth and metastasis of a murine mammary carcinoma, SP1, and a metastatic variant, SP1-3M in vivo. CBA/J female mice bearing SP1 tumor transplants were injected with anti-beta 1 integrin IgG or control nonimmune IgG (200 micrograms per mouse; i.p.) every two days. Animals received anti-CD4 antibody (100 micrograms per mouse) at time zero to suppress immunity against rabbit IgG. Outgrowth of macroscopic metastases from SP1, but not from SP1-3M primary tumors, was markedly inhibited in animals receiving anti-beta 1 integrin IgG but not nonimmune IgG. To assess the stage(s) in the metastatic cascade affected, we examined the number and diameter of micrometastatic nodules in treated and untreated groups. The diameter of micrometastases was significantly reduced in SP1-tumor-bearing mice treated with anti-beta 1 integrin IgG compared to control IgG, although the number of nodules per cm2 of lung sections examined remained unchanged. No change in the number or size of micrometastases in SP1-3M tumor-bearing mice was observed. No difference in the binding, or complement-mediated and antibody-dependent cell-mediated cytotoxicity of anti-beta 1 integrin IgG with SP1 and SP1-3M cells was detected. The results suggest that under these conditions anti-beta 1 integrin inhibits metastatic tumor growth in lung tissue, but has minimal effect on intravasation, adhesion to target organs and extravasation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|