Myoglobin entrapment in poly(vinyl alcohol) dense membranes
| Autor: | Tito Lívio Moitinho Alves, Cristiano Piacsek Borges, Kátia Cecília de Souza Figueiredo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
Vinyl alcohol Oxygen/nitrogen separation Membranes Poly(vinyl alcohol) Myoglobin General Chemical Engineering Oxygen transport chemistry.chemical_element lcsh:TP155-156 Oxygen Ferrous chemistry.chemical_compound Immobilization Membrane chemistry Chemical engineering Polymer chemistry medicine Glutaraldehyde Swelling medicine.symptom lcsh:Chemical engineering |
| Zdroj: | Brazilian Journal of Chemical Engineering, Vol 31, Iss 3, Pp 747-756 (2014) Brazilian Journal of Chemical Engineering, Volume: 31, Issue: 3, Pages: 747-756, Published: SEP 2014 Brazilian Journal of Chemical Engineering v.31 n.3 2014 Brazilian Journal of Chemical Engineering Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
| ISSN: | 0104-6632 |
| Popis: | Our goal in this study was the immobilization of myoglobin in poly(vinyl alcohol) dense membranes. Glutaraldehyde was investigated both as the crosslinking agent, aiming to increase the membrane stability in aqueous medium, and as the vehicle to bind myoglobin and PVA. Reaction and membrane synthesis were carried simultaneously in mild operating conditions in order to maintain the native protein folding. Membrane characterization comprised the water swelling degree, DSC, TGA, UV-visible spectroscopy, FTIR analysis and oxygen transport in a dialysis cell. The incorporation of myoglobin in the film decreased the water swelling degree and improved the membrane thermal properties compared to unmodified PVA membrane. The reduction of ferric iron in the prosthetic group of the protein to the ferrous form was observed. The increased affinity between oxygen and the immobilized myoglobin did not favor the release of this solute from the biocarrier. |
| Databáze: | OpenAIRE |
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