Lactobacillus ixorae sp. nov., isolated from a flower (West-Indian jasmine)
| Autor: | Chiyo Shibata, Wonnop Visessanguan, Somboon Tanasupawat, Mika Miyashita, Preeyarach Wisetkhan, Sujitra Techo, Naoto Tanaka |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Sequence analysis Jasminum Lactobacillus sanfranciscensis Flowers Peptidoglycan Microbiology 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Lactic Acid Ecology Evolution Behavior and Systematics Phylogeny Base Composition biology Strain (chemistry) Bacillus coagulans Fatty Acids food and beverages Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA Thailand Bacterial Typing Techniques Lactobacillus 030104 developmental biology chemistry Fermentation |
| Zdroj: | International journal of systematic and evolutionary microbiology. 66(12) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-stain-positive, lactic acid bacterium, strain Ru20-1T, was isolated from a flower (West-Indian jasmine) collected from Kalasin province, Thailand. A polyphasic approach was used to determine the taxonomic position of this strain. Studies of morphological and biochemical characteristics revealed that strain Ru20-1T belonged to the genus Lactobacillus . The strain was heterofermentative, non-spore-forming and rod-shaped. It produced dl-lactic acid. Based on 16S rRNA gene sequence similarity, this strain was closely related to Lactobacillus lindneri LMG 14528T (96.8 %), Lactobacillus sanfranciscensis NRIC 1548T (95.4 %) and Lactobacillus florum NRIC 0771T (95.2 %), respectively. In addition, the pheS gene sequence of strain Ru20-1T was closely related to those of L. sanfranciscensis NRIC 1548T (92.0 %), L. lindneri LMG 14528T (89.0 %) and L. florum NRIC 0771T(85.0 %). Phylogenetic analysis indicated that strain Ru20-1T was clearly separated from closely related species of the genus Lactobacillus . The DNA G+C content of strain Ru20-1T was 47.8 mol %. The cell-wall peptidoglycan type was l-Lys–d-Asp. The major cellular fatty acids were C18 : 1ω9c, C20 : 0, C20 : 1ω9c and summed feature 7 (unknown 18.846 and/or C19 : 1ω6c and/or C19 : 0 cyclo). On the basis of the data provided, strain Ru20-1T represents a novel species of the genus Lactobacillus , for which the name Lactobacillus ixorae sp. nov. is proposed. The type strain is Ru20-1T (=LMG 29008T=NBRC 111239T=PCU 346T=TISTR 2381T). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|