Biochemical characterization of binding sites for dihydropyridine and ω-conotoxin in brain of adult chicken
| Autor: | H. Hug, P. Straub, Dieter Marmé, A. Zgaga-Griesz, Ruediger Woscholski |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Dihydropyridines
Biophysics Receptors Nicotinic Peptides Cyclic Biochemistry Chromatography Affinity omega-Conotoxins chemistry.chemical_compound Affinity chromatography medicine Animals Conotoxin Binding site Molecular Biology Isradipine Chemistry Calcium channel Cell Membrane Dihydropyridine Brain Cell Biology Calcium Channel Blockers Chromatography Ion Exchange Omega-Conotoxins Receptors Neurotransmitter Kinetics Digitonin Solubility Chromatography Gel Calcium Channels Chickens medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 182:947-952 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)91823-9 |
| Popis: | Binding studies using the calcium channel blockers omega-conotoxin and dihydropyridine revealed a rather equal amount of binding sites in brain from adult chicken. The omega-conotoxin binding sites could be solubilized using digitonin, without substantial loss, whereas a great decrease in dihydropyridine binding sites was observed, indicating that both types of binding sites have different sensitivity to solubilization. In contrast to ion exchange chromatography where both binding sites comigrated, glycoprotein affinity chromatography led to a different partition of the binding sites in the flow through and eluate fractions. Our results indicate that both types of calcium channel blockers bind to different targets in adult chicken. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|