Aerosol SARS-CoV-2 in hospitals and long-term care homes during the COVID-19 pandemic

Autor: Michaeline McGuinty, Sophia den Otter-Moore, Todd Cutts, Ryan Kulka, Samantha B Kasloff, Gary Mallach, Benoit Robert, Tom Kovesi, Anand Kumar, Jay Krishnan, Bashour Yazji
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Veterinary medicine
Viral Diseases
Pulmonology
Coronaviruses
Air Microbiology
Medical Conditions
Animal Products
Chlorocebus aethiops
Materials
Pathology and laboratory medicine
Multidisciplinary
Agriculture
Medical microbiology
Method development
Hospitals
Infectious Diseases
Vesicular Stomatitis Virus
Physical Sciences
Viruses
Medicine
RNA
Viral
SARS CoV 2
Pathogens
Protein target
Research Article
Coronavirus disease 2019 (COVID-19)
SARS coronavirus
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Science
Materials Science
complex mixtures
Microbiology
Rhabdoviruses
Respiratory Disorders
Animals
Humans
Viral rna
Hospital ward
Close contact
Vero Cells
Aerosols
Medicine and health sciences
Biology and life sciences
SARS-CoV-2
Significant difference
fungi
Organisms
Viral pathogens
COVID-19
Covid 19
Long-Term Care
Aerosol
Microbial pathogens
Health Care
Health Care Facilities
Mixtures
Respiratory Infections
Environmental science
Gelatin
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 9, p e0258151 (2021)
ISSN: 1932-6203
Popis: BackgroundFew studies have quantified aerosol concentrations of SARS-CoV-2 in hospitals and long-term care homes, and fewer still have examined samples for viability. This information is needed to clarify transmission risks beyond close contact.MethodsWe deployed particulate air samplers in rooms with COVID-19 positive patients in hospital ward and ICU rooms, rooms in long-term care homes experiencing outbreaks, and a correctional facility experiencing an outbreak. Samplers were placed between 2 and 3 meters from the patient. Aerosol (small liquid particles suspended in air) samples were collected onto gelatin filters by Ultrasonic Personal Air Samplers (UPAS) fitted with 3), and with a Coriolis Biosampler over 10 minutes (total 1.5m3). Samples were assayed for viable SARS-CoV-2 virus and for the viral genome by multiplex PCR using the E and N protein target sequences. We validated the sampling methods by inoculating gelatin filters with viable vesicular stomatitis virus (VSV), and with three concentrations of viable SARS-CoV-2, operating personal samplers for 16hrs, and quantifying viable virus recovery by TCID50 assay.ResultsIn total, 138 samples were collected from 99 rooms. RNA samples were positive in 9.1% (6/66) of samples obtained with the UPAS 2.5µm samplers, 13.5% (7/52) with the UPAS 10µm samplers, and 10.0% (2/20) samples obtained with the Coriolis samplers. Culturable virus was not recovered in any samples. Viral RNA was detected in 10.9% of the rooms sampled. There was no significant difference in viral RNA recovery between the different room locations or samplers. Method development experiments indicated minimal loss of SARS-CoV-2 viability via the personal air sampler operation.Key FindingsAlthough a subset of aerosol samples exhibited detectable SARS-CoV-2 RNA at low titres, the presence of viable SARS-CoV-2 virus in aerosols appears to be infrequent at >2m distance.
Databáze: OpenAIRE
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