Functional expression and properties of Sec14p-like protein with molecular mass 45 kD from rat olfactory epithelium
| Autor: | T. N. Simonova, V. V. Radchenko, Valery M. Lipkin, Maria Merkulova, Alexander A. Bondar, T. M. Shuvaeva |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Blotting
Western Biology medicine.disease_cause Ligands Biochemistry Mass Spectrometry law.invention Olfactory mucosa Olfactory Mucosa Phosphatidylinositol Phosphates law Complementary DNA medicine Escherichia coli CRAL-TRIO domain Animals Cloning Molecular Rats Wistar Molecular mass Circular Dichroism General Medicine Molecular biology Recombinant Proteins Rats Blot Molecular Weight medicine.anatomical_structure Recombinant DNA Carrier Proteins Olfactory epithelium |
| Zdroj: | Biochemistry. Biokhimiia. 70(12) |
| ISSN: | 0006-2979 |
| Popis: | cDNA of Sec14p-like water-soluble protein with molecular mass 45 kD from rat olfactory epithelium was expressed in Escherichia coli Rosetta cells. The expression product was purified by a two-step chromatographic procedure on DEAE-Sepharose and Sephacryl S-200. The identity of structural and functional characteristics of the recombinant and native proteins was demonstrated by CD, mass spectrometry, and Western blotting. Using several lipids immobilized on nitrocellulose membranes, it was shown that phosphatidylinositol-3,4,5-triphosphate is the specific ligand for the studied protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink