High-throughput ultra-high-performance liquid chromatography/tandem mass spectrometry quantitation of insulin-like growth factor-I and leucine-rich alpha-2-glycoprotein in serum as biomarkers of recombinant human growth hormone administration
| Autor: | Richard Godfrey, Pamela Brown, Robert C. Rees, Geoffrey Goldspink, S.D. Harridge, Chris Barton, Anthony J. Blazevich, Colin S. Creaser, Phil Teale, David A. Cowan, Jane Roberts, Richard G. Kay, Cristiana P. Velloso, C. Bartlett, Graham Ball |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Chromatography Chemistry Human Growth Hormone medicine.medical_treatment Organic Chemistry Quantitative proteomics Selected reaction monitoring Tandem mass spectrometry Analytical Chemistry Insulin-like growth factor Young Adult Liquid chromatography–mass spectrometry Tandem Mass Spectrometry Standard addition medicine Biomarker (medicine) Humans Drug Monitoring Insulin-Like Growth Factor I Quantitative analysis (chemistry) Spectroscopy Chromatography High Pressure Liquid Glycoproteins |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 23(19) |
| ISSN: | 1097-0231 |
| Popis: | Insulin-like growth factor-I (IGF-I) is a known biomarker of recombinant human growth hormone (rhGH) abuse, and is also used clinically to confirm acromegaly. The protein leucine-rich alpha-2-glycoprotein (LRG) was recently identified as a putative biomarker of rhGH administration. The combination of an ACN depletion method and a 5-min ultra-high-performance liquid chromatography/tandem mass spectrometry (uHPLC/MS/MS)-based selected reaction monitoring (SRM) assay detected both IGF-I and LRG at endogenous concentrations. Four eight-point standard addition curves of IGF-I (16-2000 ng/mL) demonstrated good linearity (r(2) = 0.9991 and coefficients of variance (CVs) |
| Databáze: | OpenAIRE |
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