Dual manganese-enhanced and delayed gadolinium-enhanced MRI detects myocardial border zone injury in a pig ischemia-reperfusion model
| Autor: | Rajesh Dash, Phillip Harnish, Jaehoon Chung, Michael V. McConnell, Annett Hahn-Windgassen, Tomohiko Teramoto, Todd J. Brinton, Jennifer Lyons, Fumiaki Ikeno, Robert C. Robbins, Mihoko V. Bennett, Phillip C. Yang, Yuka Matsuura, Alan C. Yeung |
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| Rok vydání: | 2011 |
| Předmět: |
Gadolinium DTPA
Swine Gadolinium Ischemia chemistry.chemical_element Contrast Media Magnetic Resonance Imaging Cine Myocardial Reperfusion Injury Anterior Descending Coronary Artery Severity of Illness Index Article Diagnosis Differential In vivo medicine Animals Radiology Nuclear Medicine and imaging Radioisotopes Manganese Ischemic cardiomyopathy Ejection fraction medicine.diagnostic_test business.industry Myocardium Reproducibility of Results Magnetic resonance imaging medicine.disease Disease Models Animal chemistry Injections Intravenous cardiovascular system Border zone Cardiology and Cardiovascular Medicine business Nuclear medicine |
| Zdroj: | Circulation. Cardiovascular imaging. 4(5) |
| ISSN: | 1942-0080 |
| Popis: | Background— Gadolinium (Gd)-based delayed-enhancement MRI (DEMRI) identifies nonviable myocardium but is nonspecific and may overestimate nonviable territory. Manganese (Mn 2+ )-enhanced MRI (MEMRI) denotes specific Mn 2+ uptake into viable cardiomyocytes. We performed a dual-contrast myocardial assessment in a porcine ischemia-reperfusion (IR) model to test the hypothesis that combined DEMRI and MEMRI identifies viable infarct border zone (BZ) myocardium in vivo. Methods and Results— Sixty-minute left anterior descending coronary artery IR injury was induced in 13 adult swine. Twenty-one days post-IR, 3-T cardiac MRI was performed. MEMRI was obtained after injection of 0.7 mL/kg Mn 2+ contrast agent. DEMRI was then acquired after injection of 0.2 mmol/kg Gd. Left ventricular (LV) mass, infarct, and function were analyzed. Subtraction of MEMRI defect from DEMRI signal identified injured BZ myocardium. Explanted hearts were analyzed by 2,3,5-triphenyltetrazolium chloride stain and tissue electron microscopy to compare infarct, BZ, and remote myocardium. Average LV ejection fraction was reduced (30±7%). MEMRI and DEMRI infarct volumes correlated with 2,3,5-triphenyltetrazolium chloride stain analysis (MEMRI, r =0.78; DEMRI, r =0.75; P P P P >0.05). Tissue electron microscopy analysis exhibited preserved cell structure in BZ cardiomyocytes despite transmural DEMRI enhancement. Conclusions— The dual-contrast MEMRI-DEMRI detects BZ viability within DEMRI infarct zones. This approach may identify injured, at-risk myocardium in ischemic cardiomyopathy. |
| Databáze: | OpenAIRE |
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