Membrane localization of the MAK-V protein kinase

Autor:	Igor V. Korobko, S. V. Kalinichenko, E. V. Korobko
Rok vydání:	2008
Předmět:	Vesicle-associated membrane protein 8 biology Cell Membrane Cyclin-dependent kinase 2 General Medicine Cell Fractionation Biochemistry Cell Line Transport protein Cell biology Yeasts biology.protein Animals ASK1 c-Raf Protein kinase A Protein Kinases Integral membrane protein Intracellular
Zdroj:	Biochemistry (Moscow). 73:278-282
ISSN:	1608-3040 0006-2979
DOI:	10.1134/s0006297908030061
Popis:	Activities of many proteins including protein kinases are often regulated by their dynamic association with specific intracellular compartments. MAK-V is an AMPK-like protein kinase with poorly characterized functions and mechanisms of action. Similarly to many other protein kinases, association of MAK-V with specific intracellular compartments could be essential for its proper functions. In this work, we studied subcellular distribution of exogenously produced and endogenous MAK-V proteins in mammalian cells using biochemical cell fractioning aiming to supplement data on MAK-V intracellular localization studied by immunocytochemical methods. We found that a significant portion of MAK-V protein in mammalian cells is associated with membranes. Moreover, MAK-V expressed in yeast was also targeted to membrane, thus suggesting an evolutionarily conservative mechanism of MAK-V membrane association. Based on the ability of various MAK-V deletion mutants to localize to membrane and comparison of MAK-V amino acid sequences from different species, we suggest a possible mechanism governing MAK-V association with intracellular membranes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c59dd9b8fc8f512d2e036242c0998578 https://doi.org/10.1134/s0006297908030061 Zobrazit plný text záznamu Plný text ve formátu PDF