Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach
| Autor: | Yovany Moreno, Luísa N. Domingues, Monica Figueiredo, Martin L. Liebstein, Christian Epe, Felix D. Guerrero, Kylie G. Bendele, Lénaïg Halos |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_treatment Cattle Diseases Infectious and parasitic diseases RC109-216 Biology Polymerase Chain Reaction Peritrophins Immunogenicity Vaccine Haematobia irritans irritans Antigen Peritrophic matrix medicine Animals Parasites ORFS Antigens Biting flies Vaccines Immunogenicity Research Reverse vaccinology fungi Muscidae Reverse Transcription biology.organism_classification Virology Haematobia irritans Reverse transcription polymerase chain reaction Vaccinology Infectious Diseases In silico vaccine discovery Parasitology Cattle Female Adjuvant Pupariation |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 14, Iss 1, Pp 1-18 (2021) |
| ISSN: | 1756-3305 |
| Popis: | Background The horn fly, Haematobia irritans irritans, causes significant production losses to the cattle industry. Horn fly control relies on insecticides; however, alternative control methods such as vaccines are needed due to the fly's capacity to quickly develop resistance to insecticides, and the pressure for eco-friendly options. Methods We used a reverse vaccinology approach comprising three vaccine prediction and 11 annotation tools to evaluate and rank 79,542 translated open reading frames (ORFs) from the horn fly's transcriptome, and selected 10 transcript ORFs as vaccine candidates for expression in Pichia pastoris. The expression of the 10 selected transcripts and the proteins that they encoded were investigated in adult flies by reverse transcription polymerase chain reaction (RT-PCR) and mass spectrometry, respectively. Then, we evaluated the immunogenicity of a vaccine candidate in an immunization trial and the antigen’s effects on horn fly mortality and fecundity in an in vitro feeding assay. Results Six of the ten vaccine candidate antigens were successfully expressed in P. pastoris. RT-PCR confirmed the expression of all six ORFs in adult fly RNA. One of the vaccine candidate antigens, BI-HS009, was expressed in sufficient quantity for immunogenicity and efficacy trials. The IgG titers of animals vaccinated with BI-HS009 plus adjuvant were significantly higher than those of animals vaccinated with buffer plus adjuvant only from days 42 to 112, with a peak on day 56. Progeny of horn flies feeding upon blood from animals vaccinated with BI-HS009 plus adjuvant collected on day 56 had 63% lower pupariation rate and 57% lower adult emergence than the control group (ANOVA: F(1, 6) = 8.221, P = 0.028 and F(1, 6) = 8.299, P = 0.028, respectively). Conclusions The reverse vaccinology approach streamlined the discovery process by prioritizing possible vaccine antigen candidates. Through a thoughtful process of selection and in vivo and in vitro evaluations, we were able to identify a promising antigen for an anti-horn fly vaccine. Graphical abstract |
| Databáze: | OpenAIRE |
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