Molecular differences between two Jeryl Lynn mumps virus vaccine component strains, JL5 and JL2
| Autor: | W. Paul Duprex, Bert K. Rima, Phil Chambers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Jeryl Lynn
Paramyxoviridae Molecular Sequence Data Mumps Vaccine Mumps virus Biology medicine.disease_cause Virus Cell Line 03 medical and health sciences Plasmid MRNA polyadenylation Species Specificity Virology Chlorocebus aethiops medicine Animals Humans Amino Acid Sequence Mononegavirales Gene Vero Cells 030304 developmental biology Genetics 0303 health sciences Base Sequence 030306 microbiology Animal Sequence Analysis DNA biology.organism_classification DNA Viral |
| Zdroj: | The Journal of General Virology |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | The Jeryl Lynn (JL) vaccine against mumps virus (MuV) contains two components, MuVJL5 and MuVJL2, which differ by over 400 nt. Due to the occurrence of bias in the direction of mutation, these differences and those found in nucleotide sequences of different isolates of the minor component in the vaccine (MuVJL2) might be due to the effect of ADAR-like deaminases on MuV grown in tissue-cultured cells. A molecular clone of MuVJL2 (pMuVJL2) and MuVJL2-specific helper plasmids were constructed in order to investigate molecular interactions between MuVJL5 and MuVJL2, to augment the existing molecular clone of MuVJL5 (pMuVJL5) and MuVJL5-specific helper plasmids. Genome and mRNA termini of MuVJL2 were characterized, and an unusual oligo-G insertion transcriptional editing event was detected near the F mRNA polyadenylation site of MuVJL2, but not of MuVJL5. Genes encoding glycoproteins of rMuVJL2 and rMuVJL5 have been exchanged to characterize the oligo-G insertion, which associated with the specific sequence of the F gene of MuVJL2 and not with any other genes or the RNA-dependent RNA polymerase of strain MuVJL2. The results indicate that a single G-to-A sequence change obliterates the co-transcriptional editing of the F mRNA and that this oligo-G insertion does not affect the growth of the virus. |
| Databáze: | OpenAIRE |
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