Characterization, regulation, and targeting of erythroid progenitors in normal and disordered human erythropoiesis
| Autor: | Narla Mohandas, Lionel Blanc, Jimmy Hom, Anupama Narla, Brian M. Dulmovits |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cellular differentiation Population Biology Article Immunophenotyping 03 medical and health sciences hemic and lymphatic diseases Cell Self Renewal Animals Humans Immunologic Factors Erythropoiesis education Glucocorticoids Erythroid Precursor Cells Cell Proliferation education.field_of_study Cell growth Anemia Cell Differentiation Hematology Phenotype Cell biology Thalidomide 030104 developmental biology Biomarkers |
| Zdroj: | Current opinion in hematology. 24(3) |
| ISSN: | 1531-7048 |
| Popis: | The erythroid progenitors burst-forming unit-erythroid and colony-forming unit-erythroid have a critical role in erythropoiesis. These cells represent a heterogeneous and poorly characterized population with modifiable self-renewal, proliferation and differentiation capabilities. This review focuses on the current state of erythroid progenitor biology with regard to immunophenotypic identification and regulatory programs. In addition, we will discuss the therapeutic implications of using these erythroid progenitors as pharmacologic targets.Erythroid progenitors are classically characterized by the appearance of morphologically defined colonies in semisolid cultures. However, these prior systems preclude a more thorough understanding of the composite nature of progenitor populations. Recent studies employing novel flow cytometric and cell-based assays have helped to redefine hematopoiesis, and suggest that erythroid progenitors may arise from different levels of the hematopoietic tree. Moreover, the identification of cell surface marker patterns in human burst-forming unit-erythroid and colony-forming unit-erythroid enhance our ability to perform downstream functional and molecular analyses at the population and single cell level. Advances in these techniques have already revealed novel subpopulations with increased self-renewing capacity, roles for erythroid progenitors in globin gene expression, and insights into pharmacologic mechanisms of glucocorticoids and pomalidomide.Immunophenotypic and molecular characterization resolves the diversity of erythroid progenitors, and may ultimately lead to the ability to target these progenitors to ameliorate diseases of dyserythropoiesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|