Experiments with Snails Add to Our Knowledge about the Role of aPKC Subfamily Kinases in Learning
| Autor: | M. V. Roshchin, E. B. Dashinimaev, E. A. Chesnokova, Nikolay Aseyev, Peter M. Kolosov, Alena B. Zuzina, Natalia V. Bal, Pavel M. Balaban, Aliya Vinarskaya, Alexander Artyuhov |
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| Rok vydání: | 2019 |
| Předmět: |
Subfamily
Transcription Genetic 5’-RACE Snail Quantitative Biology::Cell Behavior lcsh:Chemistry lcsh:QH301-705.5 Protein Kinase C Spectroscopy Quantitative Biology::Biomolecules Quantitative Biology::Molecular Networks Translation (biology) General Medicine Computer Science Applications Cell biology Isoenzymes Multigene Family RNA splicing Helix lucorum PKMζ learning and memory Gene isoform Mathematics::Number Theory Biology Models Biological Article Catalysis Quantitative Biology::Subcellular Processes Inorganic Chemistry Structure-Activity Relationship Eukaryotic translation biology.animal Learning Protein Interaction Domains and Motifs 5′-RACE Amino Acid Sequence Physical and Theoretical Chemistry Protein kinase A mRNA isoforms Molecular Biology atypical PKCs Organic Chemistry mollusks mRNA expression biology.organism_classification Alternative Splicing lcsh:Biology (General) lcsh:QD1-999 RNA Splice Sites Snail Family Transcription Factors |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 9 International Journal of Molecular Sciences, Vol 20, Iss 9, p 2117 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20092117 |
| Popis: | Protein kinase M&zeta is considered important for memory formation and maintenance in different species, including invertebrates. PKM&zeta participates in multiple molecular pathways in neurons, regulating translation initiation rate, AMPA receptors turnover, synaptic scaffolding assembly, and other processes. Here, for the first time, we established the sequence of mRNA encoding PKM&zeta homolog in land snail Helix lucorum. We annotated important features of this mRNA: domains, putative capping sites, translation starts, and splicing sites. We discovered that this mRNA has at least two isoforms, and one of them lacks sequence encoding C1 domain. C1 deletion may be unique for snail because it has not been previously found in other species. We performed behavioral experiments with snails, measured expression levels of identified isoforms, and confirmed that their expression correlates with one type of learning. |
| Databáze: | OpenAIRE |
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